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来自糙皮侧耳的化学修饰漆酶对可溶性和纤维素结合底物的特异性

Specificities of a chemically modified laccase from Trametes hirsuta on soluble and cellulose-bound substrates.

作者信息

Schroeder M, Heumann S, Silva C J S M, Cavaco-Paulo A, Guebitz G M

机构信息

Department of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, A-8010 Graz, Austria.

出版信息

Biotechnol Lett. 2006 May;28(10):741-7. doi: 10.1007/s10529-006-9052-4. Epub 2006 May 30.

Abstract

Laccases could prevent fabrics and garments from re-deposition of dyes during washing and finishing processes by degrading the solubilized dye. However, laccase action must be restricted to solubilized dye molecules thereby avoiding decolorization of fabrics. Chemical modification of enzymes can provide a powerful tool to change the adsorption behaviour of enzymes on water insoluble polymers. Polyethylene glycol (PEG) was covalently attached onto a laccase from Trametes hirsuta. Different molecular weights of the synthetic polymer were tested in terms of adsorption behaviour and retained laccase activity. Covalent attachment of PEG onto the laccase resulted in enhanced enzyme stability while with increasing molecular weight of attached PEG the substrate affinity for the laccase conjugate decreased. The activity of the modified laccases on fibre bound dye was drastically reduced decreasing the adsorption of the enzyme on various fabrics. Compared to the 5 kDa PEG laccase conjugate (K/S value 47.60) the K/S value decreased much more (47.96-46.35) after the treatment of dyed cotton fabrics with native laccase.

摘要

漆酶可通过降解溶解的染料,防止织物和服装在洗涤和整理过程中染料重新沉积。然而,漆酶的作用必须仅限于溶解的染料分子,从而避免织物脱色。酶的化学修饰可为改变酶在水不溶性聚合物上的吸附行为提供有力工具。聚乙二醇(PEG)共价连接到毛栓菌漆酶上。测试了不同分子量的合成聚合物在吸附行为和保留漆酶活性方面的情况。PEG共价连接到漆酶上导致酶稳定性增强,而随着连接的PEG分子量增加,漆酶缀合物对底物的亲和力降低。修饰后的漆酶对纤维结合染料的活性大幅降低,减少了酶在各种织物上的吸附。与5 kDa PEG漆酶缀合物(K/S值47.60)相比,用天然漆酶处理染色棉织物后,K/S值下降得更多(从47.96降至46.35)。

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