Rodríguez Couto S, Sanromán Ma, Gübitz G M
Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain.
Chemosphere. 2005 Jan;58(4):417-22. doi: 10.1016/j.chemosphere.2004.09.033.
In this paper, the effect of redox mediators on synthetic acid dye decolourization (Sella Solid Red and Luganil Green) by laccase from Trametes hirsuta cultures has been investigated. All the redox mediators tested, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1-hydroxybenzotriazole (HBT) and Remazol Brilliant Blue R (RBBR), led to higher activities than those obtained without mediators addition showing the suitability of the laccase/mediator system (LMS) in the decolourization of acid dyes. HBT was by far the most effective mediator, showing a decolourization percentage of 88% in 10 min for Sella Solid Red and of 49% in 20 min for Luganil Green. On the other hand, the stability of laccase against several metal ions, normally found in textile wastewater, was assessed. Laccase was stable at a concentration of 1mM for 7d against all the metal ions tested except for Zn+2, CrO4(-2), Cd+2, Cr2O7(-2), Fe+2, Cu+2 and especially Hg+2. When the concentration was increased to 10mM laccase stability decreased against all the metals assayed, in particular against Fe+2. In addition, the effect of metal ions on the decolourization process was also studied. It was found that Hg+2 inhibited the dye decolourization process, being the presence of HBT absolutely required for dye decolourization.
本文研究了氧化还原介质对糙皮侧耳培养物中漆酶催化合成酸性染料（塞拉固红和卢加尼尔绿）脱色的影响。所有测试的氧化还原介质，2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、1-羟基苯并三唑（HBT）和雷马素亮蓝R（RBBR），均比未添加介质时具有更高的活性，表明漆酶/介质系统（LMS）适用于酸性染料的脱色。HBT是迄今为止最有效的介质，对塞拉固红在10分钟内脱色率达88%，对卢加尼尔绿在20分钟内脱色率达49%。另一方面，评估了漆酶对纺织废水中常见的几种金属离子的稳定性。漆酶在1mM浓度下对所有测试的金属离子（除Zn+2、CrO4(-2)、Cd+2、Cr2O7(-2)、Fe+2、Cu+2，尤其是Hg+2外）可稳定存在7天。当浓度增加到10mM时，漆酶对所有测定金属的稳定性均下降，特别是对Fe+2。此外，还研究了金属离子对脱色过程的影响。发现Hg+2抑制染料脱色过程，染料脱色绝对需要HBT的存在。
