Demchuk O N, Nyporko A Iu, Blium Ia B
Tsitol Genet. 2006 Jan-Feb;40(1):10-20.
Three-dimensional models of FtsZ-protein complexes with GDP from Arabidopsis thaliana L. localized in cytosol (Entrez database code NP190843) and in chloroplasts (Entrez database code AAA82068) were developed. Crystal structure of the FtsZ-GDP complex from archaea Methanococcus jannaschii (PDB-code 1FSZ) was used as a matrix. Secondary structures of computed models contain ten beta-strands. A chloroplast form of FtsZ-protein has ten alpha-helices and four 3(10)-helices, whereas cytosolic form of protein has nine and three structures correspondently and neither a0-helix before nucleotide-binding domain nor C-terminal 3(10)-helix in secondary domain. The T2-loop of nucleotide-binding pocket of chloroplast form of FtsZ-ptotein in position 111 contains non-charged alanin residue instead of the charged one which is typical for cytosolic and bacterial forms of proteins. At low sequence homology of FtsZ-proteins (approximately 47%) the developed models demonstrate high coincidence with matrix both in the structures of nucleotide-binding pocket and in the whole molecule. The models are completely suitable for further studies of possible sites of binding with dinitroaniline herbicides.
构建了拟南芥（Arabidopsis thaliana L.）位于细胞质（Entrez数据库代码NP190843）和叶绿体（Entrez数据库代码AAA82068）中的FtsZ - 蛋白质与GDP复合物的三维模型。以嗜甲烷球菌（Methanococcus jannaschii）的FtsZ - GDP复合物晶体结构（PDB代码1FSZ）作为模板。计算模型的二级结构包含十条β链。叶绿体形式的FtsZ - 蛋白有十条α螺旋和四条3(10)螺旋，而细胞质形式的蛋白相应地有九条和三条结构，在核苷酸结合结构域之前没有α0螺旋，在二级结构域中也没有C端3(10)螺旋。FtsZ - 蛋白叶绿体形式的核苷酸结合口袋的T2环在第111位含有不带电荷的丙氨酸残基而非带电荷残基，后者是细胞质和细菌形式蛋白的典型特征。在FtsZ - 蛋白序列同源性较低（约47%）的情况下，所构建的模型在核苷酸结合口袋结构和整个分子结构上都与模板高度吻合。这些模型完全适用于进一步研究与二硝基苯胺除草剂可能的结合位点。
