Goya L, Timiras P S
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Neurochem Res. 1991 Feb;16(2):113-6. doi: 10.1007/BF00965697.
Nuclear receptors for the thyroid hormone triiodothyronine (T3) have been identified in vivo in brain tissues and in vitro in mouse and rat neuroblastoma and glioma cells. The present study characterizes nuclear T3 receptors in human neuroblastoma SH-SY5Y cells and compares their level before and after differentiation. Undifferentiated cells, grown in DME/HAM F-12 medium supplemented with 10% fetal calf serum, show an abundant single type of nuclear receptor, indicated by a straight Scatchard plot, with a Kd of 0.11 nmol/l. After treatment with sodium butyrate (0.5 mM for 4 days) or NGF (2 nM for 6 days), the cells showed neuronal-like patterns (extension of neurites, slowing of growth, increased tyrosine hydroxylase activity), with a decrease in the number of nuclear T3 receptors. As sodium butyrate and NGF treatments differentiate neuroblastoma SH-SY5Y cells, these data suggest a down-regulation of T3 receptors with cell maturation.
甲状腺激素三碘甲状腺原氨酸（T3）的核受体已在脑组织中被体内鉴定，在小鼠和大鼠神经母细胞瘤及胶质瘤细胞中被体外鉴定。本研究对人神经母细胞瘤SH-SY5Y细胞中的核T3受体进行了表征，并比较了其分化前后的水平。在补充有10%胎牛血清的DME/HAM F-12培养基中生长的未分化细胞，显示出一种丰富的单一类型核受体，由直线型Scatchard图表明，其解离常数（Kd）为0.11 nmol/l。在用丁酸钠（0.5 mM，处理4天）或神经生长因子（NGF，2 nM，处理6天）处理后，细胞呈现出神经元样模式（神经突延长、生长减慢、酪氨酸羟化酶活性增加），同时核T3受体数量减少。由于丁酸钠和NGF处理可使神经母细胞瘤SH-SY5Y细胞分化，这些数据表明随着细胞成熟，T3受体下调。
