Jansen K L, Dragunow M, Faull R L, Leslie R A
MRC Unit, Radcliffe Infirmary, Oxford, U.K.
Neurosci Lett. 1991 Apr 29;125(2):143-6. doi: 10.1016/0304-3940(91)90012-i.
The autoradiographic distributions of [3H]1,3-di-ortho-tolyguanidine ([3H]DTG), [3H]1-[1-(2-thienyl) cyclohexyl] piperidine ([3H]TCP) and L-[3H]glutamate were studied in the human cerebellum. [3H]DTG is a selective label for the sigma receptor, while L-[3H]glutamate binding was carried out under conditions selective for the N-methyl-D-aspartate (NMDA) receptor. [3H]TCP binding sites and sigma receptors showed marked enrichment in the Purkinje cell layer, while L-[3H]glutamate-labelled NMDA receptors showed virtually no binding in the Purkinje cell layer. The results confirm the existence of [3H]TCP binding sites which are not linked to NMDA receptors in the human cerebellum, having a distribution which is more similar to that of the haloperidol-sensitive sigma receptor.
研究了[3H]1,3 - 二邻甲苯基胍（[3H]DTG）、[3H]1 - [1 - (2 - 噻吩基)环己基]哌啶（[3H]TCP）和L - [3H]谷氨酸在人小脑的放射自显影分布。[3H]DTG是σ受体的选择性标记物，而L - [3H]谷氨酸结合是在对N - 甲基 - D - 天冬氨酸（NMDA）受体有选择性的条件下进行的。[3H]TCP结合位点和σ受体在浦肯野细胞层有明显富集，而L - [3H]谷氨酸标记的NMDA受体在浦肯野细胞层几乎没有结合。结果证实人小脑中存在不与NMDA受体相连的[3H]TCP结合位点，其分布与对氟哌啶醇敏感的σ受体更为相似。
