氯化铝刺激培养的大鼠皮质星形胶质细胞释放内源性谷氨酸、牛磺酸和腺苷。

Aluminum chloride stimulates the release of endogenous glutamate, taurine and adenosine from cultured rat cortical astrocytes.

作者信息

Albrecht J, Simmons M, Dutton G R, Norenberg M D

机构信息

Veteran Administration Medical Center, Department of Pathology, Miami, FL.

出版信息

Neurosci Lett. 1991 Jun 10;127(1):105-7. doi: 10.1016/0304-3940(91)90905-9.

DOI:10.1016/0304-3940(91)90905-9

PMID:1679221

Abstract

Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.

摘要

将新生大鼠大脑皮层来源的原代星形胶质细胞培养物用0.5 mM或5.0 mM的AlCl₃处理5分钟，然后通过高效液相色谱法分析孵育培养基中释放的谷氨酸（Glu）、牛磺酸（Tau）、丝氨酸（Ser）和核苷腺苷（Ade）的含量。在0.5 mM时，AlCl₃刺激Tau释放至基础水平的约170%，但不影响其他化合物的释放。用5.0 mM AlCl₃处理可使Tau、Glu和Ade的释放分别增加至800%、1000%和250%，但与基础水平相比，Ser的释放减少至70%。这些神经活性化合物从星形胶质细胞的释放增加可能导致已知的与铝暴露相关的神经传递变化。