Pelletier G, Simard J
MRC Group in Molecular Endocrinology, Laval University Medical Center, Québec, Que. Canada.
Neurosci Lett. 1991 Jun 10;127(1):96-8. doi: 10.1016/0304-3940(91)90903-7.
In order to study the role of dopamine on neuropeptide Y (NPY) gene expression in the rat arcuate nucleus, we evaluated the effects of haloperidol, a dopaminergic antagonist, and bromocriptine, a D2 dopamine receptor agonist, on the levels of pre-proNPY mRNA as measured by in situ hybridization. Chronic treatment with bromocriptine produced a moderate decrease of the hybridization signal, whereas haloperidol treatment resulted in a marked increase in signal intensities. These results indicate that, in the arcuate nucleus, NPY neurons are negatively regulated by dopaminergic-mediated mechanisms acting most likely through D2 receptors.
为了研究多巴胺对大鼠弓状核中神经肽Y（NPY）基因表达的作用，我们通过原位杂交技术评估了多巴胺能拮抗剂氟哌啶醇和D2多巴胺受体激动剂溴隐亭对前体NPY mRNA水平的影响。长期使用溴隐亭治疗会使杂交信号适度降低，而氟哌啶醇治疗则导致信号强度显著增加。这些结果表明，在弓状核中，NPY神经元受到多巴胺能介导机制的负调控，这种机制很可能通过D2受体起作用。
