Chen J J, Throop M S, Gehrke L, Kuo I, Pal J K, Brodsky M, London I M
Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge.
Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7729-33. doi: 10.1073/pnas.88.17.7729.
We have cloned the cDNA of the heme-regulated eIF-2 alpha kinase (HRI) of rabbit reticulocytes. In vitro translation of mRNA transcribed from the HRI cDNA yields a 90-kDa polypeptide that exhibits eIF-2 alpha kinase activity and is recognized by a monoclonal antibody directed against authentic HRI. The open reading frame sequence of the HRI cDNA contains all 11 catalytic domains of protein kinases with consensus sequences of protein-serine/threonine kinases in conserved catalytic domains VI and VIII. The HRI cDNA also contains an insert of approximately 140 amino acids between catalytic domains V and VI. The HRI cDNA coding sequence has extensive homology to GCN2 protein kinase of Saccharomyces cerevisiae and to human double-stranded-RNA-dependent eIF-2 alpha kinase. This observation suggests that GCN2 protein kinase may be an eIF-2 alpha kinase in yeast. In addition, HRI has an unusually high degree of homology to three protein kinases (NimA, Wee1, and CDC2) that are involved in the regulation of the cell cycle.
我们已经克隆了兔网织红细胞血红素调节性真核起始因子2α激酶（HRI）的cDNA。从HRI cDNA转录的mRNA在体外翻译产生一种90 kDa的多肽，该多肽具有真核起始因子2α激酶活性，并被针对天然HRI的单克隆抗体识别。HRI cDNA的开放阅读框序列包含蛋白激酶的所有11个催化结构域，在保守的催化结构域VI和VIII中具有蛋白丝氨酸/苏氨酸激酶的共有序列。HRI cDNA在催化结构域V和VI之间还包含一个约140个氨基酸的插入片段。HRI cDNA编码序列与酿酒酵母的GCN2蛋白激酶和人双链RNA依赖性真核起始因子2α激酶具有广泛的同源性。这一观察结果表明，GCN2蛋白激酶可能是酵母中的一种真核起始因子2α激酶。此外，HRI与参与细胞周期调控的三种蛋白激酶（NimA、Wee1和CDC2）具有异常高的同源性。
