Trifaro J M, Dworkind J
Can J Physiol Pharmacol. 1975 Jun;53(3):479-92. doi: 10.1139/y75-068.
Adenosine triphosphate (ATP) induces the release of catecholamines, endogenous ATP, and soluble protein from chromaffin granules isolated from the adrenal medulla. When ATP exerts this action, it is hydrolyzed by enzymes present in the granule membrane, and part of the Pi liberated from ATP is transferred to the protein and lipid of the granule membrane. The phosphorylated lipid component, which was identified by thin-layer and ion-exchange chromatography as diphosphatidylinositol, was formed from ATP and monophosphatidylinositol. This latter phospholipid was the substrate for the enzyme phosphatidylinositol kinase. Both substrate and enzyme are components of the granule membranes, because they have a similar subcellular distribution as dopamine beta-hydroxylase (a granule membrane marker). The formation of diphosphatidylinositol was Mg(2 plus)-dependent, it was further stimulated by Mn(2 plus), it was inhibited by N-ethylmaleimide and the reaction had an optimal pH of 5. The synthesis of diphosphatidylinositol was also shown to occur in chromaffin granules "in situ". during the stimulation of the adrenal medulla by acetylcholine.
三磷酸腺苷（ATP）可诱导从肾上腺髓质分离出的嗜铬颗粒释放儿茶酚胺、内源性ATP和可溶性蛋白质。当ATP发挥这种作用时，它会被颗粒膜中存在的酶水解，并且从ATP释放的部分磷酸根会转移到颗粒膜的蛋白质和脂质上。通过薄层色谱和离子交换色谱鉴定为二磷脂酰肌醇的磷酸化脂质成分，是由ATP和单磷脂酰肌醇形成的。后一种磷脂是磷脂酰肌醇激酶的底物。底物和酶都是颗粒膜的成分，因为它们与多巴胺β-羟化酶（一种颗粒膜标记物）具有相似的亚细胞分布。二磷脂酰肌醇的形成依赖于Mg（2+），可被Mn（2+）进一步刺激，受N-乙基马来酰亚胺抑制，反应的最适pH为5。二磷脂酰肌醇的合成也显示在嗜铬颗粒“原位”发生，即在乙酰胆碱刺激肾上腺髓质期间。
