Grüber C, Gerhold K, von Stuckrad S L, Avagyan A, Quarcoo D, Ahrens B, Wahn U, Hamelmann E
Pediatric Pneumology and Immunology, Charité-Universitätsmedizin Berlin, Germany.
Allergy. 2006 Jul;61(7):820-7. doi: 10.1111/j.1398-9995.2006.01093.x.
Co-vaccination with cellular pertussis vaccine down-regulates allergic sensitization to diphtheria and tetanus antigens. Using a murine model, we investigated whether vaccination with diphtheria/tetanus toxoids, administered separately or simultaneously with the whole cell vaccine of Bordetella pertussis, inhibits subsequent allergen-induced immune and inflammatory responses.
BALB/c-mice were vaccinated intracutaneously with a combination of diphtheria and tetanus toxoids or a combination of diphtheria and tetanus toxoids with a whole cell vaccine of B. pertussis (three times, days -21 to -7) prior to systemic sensitization (days 1-14) and repeated airway challenges (days 28-30) with ovalbumin.
Compared with negative controls, systemic sensitization and airway allergen challenges induced high serum levels of allergen-specific IgE, predominant Th2-type cytokine production, airway inflammation and development of in vivo airway hyperreactivity. Vaccination with diphtheria and tetanus toxoids prior to sensitization suppressed IgE formation and development of eosinophilic airway inflammation. Co-vaccination with a whole cell pertussis vaccine inhibited allergen sensitization, airway inflammation and development of in vivo airway hyperreactivity. Prevention was due to an allergen-specific and general shift from a predominant Th2 towards a predominant Th1 immune response.
Vaccination with diphtheria and tetanus toxoids alone or in combination with whole cell pertussis vaccine prior to allergen sensitization prevented allergen-induced Th2 immune responses. Vaccine antigens may down-regulate allergic responses to a range of common allergens.
与细胞百日咳疫苗联合接种可下调对白喉和破伤风抗原的过敏致敏反应。我们使用小鼠模型研究了接种白喉/破伤风类毒素，单独接种或与百日咳博德特氏菌全细胞疫苗同时接种，是否能抑制随后变应原诱导的免疫和炎症反应。
在系统性致敏（第1 - 14天）和用卵清蛋白进行反复气道激发（第28 - 30天）之前，BALB/c小鼠经皮内接种白喉和破伤风类毒素组合或白喉和破伤风类毒素与百日咳博德特氏菌全细胞疫苗的组合（共三次，第 - 21天至 - 7天）。
与阴性对照相比，系统性致敏和气道变应原激发诱导了高血清水平的变应原特异性IgE、主要的Th2型细胞因子产生、气道炎症以及体内气道高反应性的发展。在致敏前接种白喉和破伤风类毒素可抑制IgE形成和嗜酸性气道炎症的发展。与全细胞百日咳疫苗联合接种可抑制变应原致敏、气道炎症以及体内气道高反应性的发展。预防是由于变应原特异性以及从主要的Th2免疫反应向主要的Th1免疫反应的总体转变。
在变应原致敏前单独接种白喉和破伤风类毒素或与全细胞百日咳疫苗联合接种可预防变应原诱导的Th2免疫反应。疫苗抗原可能下调对一系列常见变应原的过敏反应。
