Chamayou S, Alecci C, Ragolia C, Storaci G, Maglia E, Russo E, Guglielmino A
Unità di Medicina della Riproduzione - Fondazione HERA, Viale Marco Polo 39/A, 95126 Catania, Italy.
Reprod Biomed Online. 2006 Jun;12(6):730-6. doi: 10.1016/s1472-6483(10)61085-1.
In Italy, the restrictive IVF law generalizes the indication for oocyte freezing for surplus oocytes in 78.5% of in-vitro assisted reproductive cycles. With a view to understanding better what the prospects for intracytoplasmic sperm injection (ICSI) on frozen-thawed oocytes might be, the consequences of freeze-thaw procedures on fertilization, cleavage rates and embryo quality obtained from frozen-thawed oocytes were studied and compared with the results obtained from sibling fresh oocytes. Eleven IVF and 29 ICSI on 76 and 169 fresh oocytes were performed and the corresponding 40 ICSI on 221 sibling frozen-thawed oocytes. There was no difference in terms of fertilization rate between fresh and sibling frozen-thawed oocytes. The cleavage rate (98.0 and 94.4% with fresh oocytes in IVF and ICSI; 77.3% with frozen-thawed oocytes in ICSI; P < 0.001) and embryo quality (grade I embryos over total embryos: 36.7 and 22.2% with fresh oocytes in IVF and ICSI; 12.1% with frozen-thawed oocytes in ICSI; respectively P < 0.001 and P < 0.05) were statistically lower after oocyte cryopreservation. The significant decrease in meiotic spindle retrieval rate before freezing (62.4%) and after thawing procedures (43.4%; P < 0.001) suggests that cryoconservation induces irreversible damage to microtubule repolymerization. The consequences of oocyte cryopreservation procedures on embryo development are reviewed.
在意大利，严格的体外受精（IVF）法律规定，在78.5%的体外辅助生殖周期中，多余的卵母细胞都要进行冷冻保存。为了更好地了解冻融卵母细胞的胞浆内单精子注射（ICSI）前景，研究了冻融程序对冻融卵母细胞受精、卵裂率和胚胎质量的影响，并与同期新鲜卵母细胞的结果进行了比较。对76个和169个新鲜卵母细胞分别进行了11次IVF和29次ICSI，对221个同期冻融卵母细胞进行了相应的40次ICSI。新鲜卵母细胞和同期冻融卵母细胞的受精率没有差异。卵母细胞冷冻保存后，卵裂率（IVF和ICSI中新鲜卵母细胞分别为98.0%和94.4%；ICSI中冻融卵母细胞为77.3%；P<0.001）和胚胎质量（I级胚胎占总胚胎的比例：IVF和ICSI中新鲜卵母细胞分别为36.7%和22.2%；ICSI中冻融卵母细胞为12.1%；分别为P<0.001和P<0.05）在统计学上较低。冷冻前（62.4%）和解冻后（43.4%；P<其显著降低表明冷冻保存会对微管重新聚合造成不可逆的损害。本文综述了卵母细胞冷冻保存程序对胚胎发育的影响。 001）减数分裂纺锤体回收率的显著下降表明冷冻保存会对微管重新聚合造成不可逆的损害。本文综述了卵母细胞冷冻保存程序对胚胎发育的影响。
