HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan.
Reproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan.
Biomolecules. 2022 Jul 21;12(7):1008. doi: 10.3390/biom12071008.
Although it is not a well-established technology, oocyte cryopreservation is becoming prevalent in assisted reproductive technologies in response to the growing demands of patients' sociological and pathological conditions. Oocyte cryopreservation can adversely affect the developmental potential of oocytes by causing an increase in intracellular oxidative stresses and damage to the mitochondrial structure. In this study, we studied whether autologous adipose stem cell (ASC) mitochondria supplementation with vitrified and warmed oocytes could restore post-fertilization development that decreased due to mitochondrial damage following cryopreservation. ASC mitochondria showed similar morphology to oocytes' mitochondria and had a higher ATP production capacity. The vitrified-warmed oocytes from juvenile mice were supplemented with ASC mitochondria at the same time as intracellular sperm injection (ICSI), after which we compared their developmental capacity and the mitochondria quality of 2-cell embryos. We found that, compared to their counterpart, mitochondria supplementation significantly improved development from 2-cell embryos to blastocysts (56.8% vs. 38.2%) and ATP production in 2-cell embryos (905.6 & 561.1 pmol), while reactive oxygen species levels were comparable. With these results, we propose that ASC mitochondria supplementation could restore the quality of cryopreserved oocytes and enhance the embryo developmental capacity, signifying another possible approach for mitochondrial transplantation therapy.
虽然卵母细胞冷冻保存技术尚未得到广泛认可，但为了满足患者社会学和病理学条件的不断增长的需求，它在辅助生殖技术中越来越流行。卵母细胞冷冻保存会通过增加细胞内氧化应激和破坏线粒体结构来降低卵母细胞的发育潜能。在这项研究中，我们研究了是否可以通过补充自体脂肪干细胞（ASC）线粒体来恢复因冷冻保存导致的线粒体损伤而降低的受精后发育。ASC 线粒体与卵母细胞的线粒体具有相似的形态，并且具有更高的 ATP 产生能力。同时将 ASC 线粒体与体内精子注射（ICSI）一起添加到来自幼年小鼠的玻璃化-加热卵母细胞中，然后比较它们的发育能力和 2 细胞胚胎的线粒体质量。我们发现，与对照组相比，线粒体补充可显著提高从 2 细胞胚胎到囊胚的发育（56.8%比 38.2%）和 2 细胞胚胎中的 ATP 产生（905.6 和 561.1 pmol），而活性氧水平相当。有了这些结果，我们提出 ASC 线粒体补充可以恢复冷冻保存卵母细胞的质量并增强胚胎发育能力，这表明了另一种可能的线粒体移植治疗方法。
