Terry Claire, Dhawan Anil, Mitry Ragai R, Hughes Robin D
King's College London School of Medicine at King's College Hospital, Institute of Liver Studies, Bessemer Road, London SE5 9PJ, UK.
Cryobiology. 2006 Oct;53(2):149-59. doi: 10.1016/j.cryobiol.2006.05.004. Epub 2006 Jun 21.
Hepatocytes isolated from unused donor livers are being used for transplantation in patients with acute liver failure and liver-based metabolic defects. As large numbers of hepatocytes can be prepared from a single liver and hepatocytes need to be available for emergency and repeated treatment of patients it is essential to be able to cryopreserve and store cells with good thawed cell function. This review considers the current status of cryopreservation of human hepatocytes discussing the different stages involved in the process. These include pre-treatment of cells, freezing solution, cryoprotectants and freezing and thawing protocols. There are detrimental effects of cryopreservation on hepatocyte structure and metabolic function, including cell attachment, which is important to the engraftment of transplanted cells in the liver. Cryopreserved human hepatocytes have been successfully used in clinical transplantation, with evidence of replacement of missing function. Further optimisation of hepatocyte cryopreservation protocols is important for their use in hepatocyte transplantation.
从未使用过的供体肝脏中分离出的肝细胞正被用于急性肝衰竭和肝脏代谢缺陷患者的移植。由于从单个肝脏中可以制备大量肝细胞，且肝细胞需要可用于患者的紧急和重复治疗，因此能够冷冻保存并储存具有良好解冻后细胞功能的细胞至关重要。本综述考虑了人肝细胞冷冻保存的现状，讨论了该过程中涉及的不同阶段。这些阶段包括细胞预处理、冷冻溶液、冷冻保护剂以及冷冻和解冻方案。冷冻保存对肝细胞结构和代谢功能有不利影响，包括细胞附着，而细胞附着对移植细胞在肝脏中的植入很重要。冷冻保存的人肝细胞已成功用于临床移植，有证据表明其缺失的功能得到了替代。进一步优化肝细胞冷冻保存方案对其在肝细胞移植中的应用很重要。
