一种用于诊断胆管癌的血清MUC5AC粘蛋白的新型粘蛋白抗体/酶联凝集素夹心测定法。

A new mucin antibody/enzyme-linked lectin-sandwich assay of serum MUC5AC mucin for the diagnosis of cholangiocarcinoma.

作者信息

Bamrungphon Wichuda, Prempracha Nalinee, Bunchu Nophawan, Rangdaeng Samreung, Sandhu Trichak, Srisukho Songphol, Boonla Chanchai, Wongkham Sopit

机构信息

Department of Microbiology, Faculty of Medicine, Chiang Mai Universtiy, Chiang Mai 50002, Thailand.

出版信息

Cancer Lett. 2007 Mar 18;247(2):301-8. doi: 10.1016/j.canlet.2006.05.007. Epub 2006 Jun 21.

DOI:10.1016/j.canlet.2006.05.007

PMID:16793202

Abstract

We have previously used agarose gel electrophoresis and immunoblotting to qualitatively measure serum MUC5AC mucin for diagnosing cholangiocarcinoma. In this study, we developed a quantitative determination of serum MUC5AC by sandwich ELISA using MUC5AC mucin monoclonal antibody and soybean agglutinin. A cut-off value of the absorbance 0.074 was obtained from a complete statistical Receiver Operating Characteristic curves with an area under the curve=0.8141. The assay could discriminate cholangiocarcinoma patients from the controls with 71% sensitivity and 90% specificity. The test is simple to perform, reproducible, and probably used for detecting cholangiocarcinoma in a high-risk group or suspected patients.

摘要

我们之前曾使用琼脂糖凝胶电泳和免疫印迹法定性检测血清MUC5AC黏蛋白以诊断胆管癌。在本研究中，我们使用MUC5AC黏蛋白单克隆抗体和大豆凝集素，通过夹心酶联免疫吸附测定法（ELISA）对血清MUC5AC进行了定量测定。通过完整的统计受试者工作特征曲线获得吸光度截断值为0.074，曲线下面积=0.8141。该检测方法能够以71%的灵敏度和90%的特异性区分胆管癌患者与对照组。该检测操作简单、可重复，可能用于在高危人群或疑似患者中检测胆管癌。