血清MUC5AC粘蛋白作为胆管癌的潜在标志物。

Serum MUC5AC mucin as a potential marker for cholangiocarcinoma.

作者信息

Wongkham Sopit, Sheehan John Kieran, Boonla Chanchai, Patrakitkomjorn Siriporn, Howard Marjorie, Kirkham Sara, Sripa Banchob, Wongkham Chaisiri, Bhudhisawasdi Vajarabhongsa

机构信息

Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Cancer Lett. 2003 May 30;195(1):93-9. doi: 10.1016/s0304-3835(02)00691-2.

DOI:10.1016/s0304-3835(02)00691-2

PMID:12767517

Abstract

Aberrant expression of MUC5AC mucin is obvious in cholangiocarcinoma tissues, however, this mucin has never been detected in the serum. Using immunoblotting marked with antibody vs. MUC5AC core protein, we could detect MUC5AC mucin in the serum of 112 from 179 cholangiocarcinoma patients (62.6% sensitivity), two of the 62 with benign hepatobiliary diseases, six of the 60 with hepato-gastrointestinal cancer, and none in either the 60 active opisthorchiasis or 74 healthy persons. Detection of serum mucin in the serum of cholangiocarcinoma patients corresponded well to the MUC5AC expressed in individual tissues. Serum MUC5AC may be used to enhance the diagnostic accuracy of cholangiocarcinoma.

摘要

MUC5AC粘蛋白在胆管癌组织中存在异常表达，然而，在血清中从未检测到这种粘蛋白。使用针对MUC5AC核心蛋白的抗体进行免疫印迹分析，我们在179例胆管癌患者中的112例血清中检测到了MUC5AC粘蛋白（敏感性为62.6%），62例良性肝胆疾病患者中有2例，60例肝胃肠癌患者中有6例，而60例活动性华支睾吸虫病患者和74例健康人中均未检测到。胆管癌患者血清中粘蛋白的检测结果与个体组织中表达的MUC5AC情况高度相符。血清MUC5AC可用于提高胆管癌的诊断准确性。