[端粒酶逆转录酶、c-myc、增殖细胞核抗原表达及细胞凋亡在肝癌发生中的作用]

[Expressions of h-TERT, c-myc, PCNA and cell apoptosis in liver carcinogenesis].

作者信息

Fu Xiao-mei, Yang Qing-xu, Shao Chun-kui, Feng Zhi-ying

机构信息

Department of Infectious Diseases, Central Peoples' Hospital of Huizhou City, Huizhou 516001, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jun;26(6):821-3.

PMID:16793609

Abstract

OBJECTIVE

To investigate the expressions of human telomerase reverse transcriptase (h-TERT), c-myc, and proliferating cell nuclear antigen (PCNA) in chronic viral hepatitis (CVH), liver cirrhosis and primary hepatocellular carcinoma (HCC) and understand their possible role in liver carcinogenesis.

METHODS

Totally 157 liver disease specimens were collected, including 56 CVH, 52 liver cirrhosis and 49 primary HCC specimens. In situ hybridization was performed on these specimens to examine the expressions of h-TRET and c-myc mRNA, and immunohistochemistry carried out for PCNA detection, with the cell apoptosis detected with in situ ending labeling.

RESULTS

In the CVH, liver cirrhosis and primary HCC specimens, h-TERT expression was detected at the frequencies of 11/56 (19.6%), 43/52 (82.7%) and 44/47 (93.6%), c-myc expression at 7/56 (12.5%), 21/52 (40.4%) and 26/47 (55.3%), with apoptotic index of (27.3-/+4.7)%, (16.5-/+2.6)% and (8.7-/+1.3)% and PCNA expression rate of (17.1-/+2.9)%, (49.3-/+7.8)% and (62.5-/+9.1)%, respectively. Correlations among h-TERT, c-myc, and PCNA expressions and the apoptotic index were not found in the examined tissues (P>0.05).

CONCLUSION

Liver carcinogenesis may involve increased h-TERT, c-myc, and PCNA expressions and suppressed cell apoptosis.

摘要

目的

研究人类端粒酶逆转录酶（h-TERT）、c-myc和增殖细胞核抗原（PCNA）在慢性病毒性肝炎（CVH）、肝硬化及原发性肝细胞癌（HCC）中的表达情况，并了解它们在肝癌发生过程中可能发挥的作用。

方法

共收集157份肝病标本，其中包括56份CVH标本、52份肝硬化标本和49份原发性HCC标本。对这些标本进行原位杂交以检测h-TRET和c-myc mRNA的表达，采用免疫组织化学法检测PCNA，并用原位末端标记法检测细胞凋亡。

结果

在CVH、肝硬化和原发性HCC标本中，h-TERT表达的检出率分别为11/56（19.6%）、43/52（82.7%）和44/47（93.6%）；c-myc表达的检出率分别为7/56（12.5%）、21/52（40.4%）和26/47（55.3%）；凋亡指数分别为（27.3±4.7）%、（16.5±2.6）%和（8.7±1.3）%；PCNA表达率分别为（17.1±2.9）%、（49.3±7.8）%和（62.5±9.1）%。在所检测的组织中，未发现h-TERT、c-myc和PCNA表达与凋亡指数之间存在相关性（P>0.05）。