Menshanov Petr N, Bannova Anita V, Dygalo Nikolay N
Functional Neurogenomics Laboratory, Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, Russian Federation.
Neurochem Res. 2006 Jul;31(7):869-75. doi: 10.1007/s11064-006-9089-8. Epub 2006 Jun 21.
DNA fragmentation, mRNA and protein levels of Bcl-XL, Bax and caspase-3 were determined to characterize interrelations between expression of these apoptotic markers in the neonatal brain regions. High DNA fragmentation intensity in the cortex was in consonance with the lowest Bcl-XL/Bax expression ratio, the highest procaspase-3 and active caspase-3 levels. Low and intermediate DNA fragmentation levels in the cerebellum and hippocampus respectively were also in a good agreement with apoptotic proteins expression in these structures. In the cortex, hippocampus and cerebellum DNA fragmentation intensity was proportional to the active caspase-3 level. In contrast to these structures, in the brainstem, the lowest level of this protease was accompanied by the highest intensity of DNA fragmentation among the brain regions studied. The data suggest that cell death normally occurring during early postnatal life could be realized in the developing brainstem via caspase-3-independent pathways in animals that express this protease.
测定DNA片段化、Bcl-XL、Bax和caspase-3的mRNA及蛋白水平，以表征这些凋亡标志物在新生动物脑区的表达之间的相互关系。皮质中高DNA片段化强度与最低的Bcl-XL/Bax表达比率、最高的procaspase-3和活性caspase-3水平一致。小脑和海马体中分别较低和中等的DNA片段化水平也与这些结构中凋亡蛋白的表达高度吻合。在皮质、海马体和小脑中，DNA片段化强度与活性caspase-3水平成正比。与这些结构相反，在脑干中，在所研究的脑区中，这种蛋白酶的最低水平伴随着最高的DNA片段化强度。数据表明，在出生后早期正常发生的细胞死亡，在表达这种蛋白酶的动物中，可能通过caspase-3非依赖途径在发育中的脑干中实现。
