Sendow I, Daniels P W, Cybinski D H, Young P L, Ronohardjo P
Research Institute for Veterinary Science, Bogor, Indonesia.
Vet Microbiol. 1991 Jun;28(1):111-8. doi: 10.1016/0378-1135(91)90103-m.
The orbiviruses contain several important viruses of livestock including bluetongue (BT) and epizootic haemorrhagic disease of deer (EHD) which share some group antigens. Preliminary screening of sera for antibodies to orbiviruses by the agar gel immunodiffusion (AGID) test has previously revealed widespread infections with the BT group in Indonesia. However serum neutralization (SN) tests give a more accurate estimate of exposure to each serotype in the BT and EHD groups, and in this study were applied to sera that had reacted previously in the AGID test. Five different serotypes of BT and one serotype of EHD virus were studied. Reactors to BT serotype 20 were the most prevalent, followed by EHD type 5 and BT types 21, 12, 1 and 17. Antibodies against BT serotype 20 were present in cattle, buffaloes, goats and sheep, but were most common in buffaloes. Buffaloes showed the highest exposure to the BT serotypes tested. Antibody to EHD type 5 occurred most frequently in cattle. Antibodies against all BT and EHD serotypes tested were found in buffaloes and cattle while goats had antibodies against BT types 20, 21 and EHD type 5 and sheep had antibodies only against BT type 20.
环状病毒包含几种重要的家畜病毒，包括蓝舌病（BT）和鹿流行性出血病（EHD），它们有一些共同的群抗原。此前通过琼脂凝胶免疫扩散（AGID）试验对血清进行环状病毒抗体的初步筛查发现，印度尼西亚存在广泛的蓝舌病病毒组感染。然而，血清中和（SN）试验能更准确地估计蓝舌病和鹿流行性出血病病毒组中每种血清型的暴露情况，在本研究中，该试验应用于先前在AGID试验中呈阳性反应的血清。研究了五种不同血清型的蓝舌病病毒和一种血清型的鹿流行性出血病病毒。对蓝舌病血清型20呈阳性反应的最为普遍，其次是鹿流行性出血病病毒5型以及蓝舌病病毒21型、12型、1型和17型。牛、水牛、山羊和绵羊体内均存在针对蓝舌病血清型20的抗体，但在水牛中最为常见。水牛对所检测的蓝舌病血清型的暴露率最高。鹿流行性出血病病毒5型抗体在牛中出现的频率最高。在所检测的所有蓝舌病和鹿流行性出血病血清型中，水牛和牛体内均发现了抗体，而山羊体内有针对蓝舌病血清型20、21和鹿流行性出血病病毒5型的抗体，绵羊体内仅含有针对蓝舌病血清型20的抗体。
