Bücking W, Nann T
Freiburg Materials Research Center (FMF), Albert-Ludwig University Freiburg, Germany.
IEE Proc Nanobiotechnol. 2006 Jun;153(3):47-53. doi: 10.1049/ip-nbt:20050043.
Efforts were made to realise a two-dimensional, on-line-coupled isotachophoresis-capillary zone electrophoresis system. The electrophoretic behaviour of gold nanoparticles was investigated with the idea that they could be used to improve the control of this electrophoretic set-up. The well-known citrate-ligated gold nanoparticles were not suitable for this application, because the ligand was desorbed, and the nanoparticle solutions were degraded. Therefore mercaptocarboxylic acids were used, because the chemisorption of thiols on the gold surface was improved. Isotachophoretic measurements were carried out with these nanoparticles. A size-dependent electrophoretic mobility was found according to theoretical predictions, and the surface and zeta-potential were discussed for the small particle range. A new method for concentration measurements of nanoparticles is presented by means of isotachophoresis.
人们致力于实现一种二维在线耦合等速电泳 - 毛细管区带电泳系统。研究了金纳米颗粒的电泳行为，其思路是它们可用于改善对这种电泳装置的控制。众所周知，柠檬酸盐连接的金纳米颗粒不适用于此应用，因为配体解吸，且纳米颗粒溶液降解。因此使用了巯基羧酸，因为硫醇在金表面的化学吸附得到了改善。用这些纳米颗粒进行了等速电泳测量。根据理论预测发现了尺寸依赖性电泳迁移率，并讨论了小颗粒范围内的表面和zeta电位。通过等速电泳提出了一种测量纳米颗粒浓度的新方法。
