De Omena M C, Bento E S, De Paula J E, Sant'Ana A E G
Departamento de Química, Universidade Federal de Alagoas, Maceió-AL, Brazil.
Vector Borne Zoonotic Dis. 2006 Summer;6(2):216-22. doi: 10.1089/vbz.2006.6.216.
Ethanolic extract from seeds of Pterodon polygalaeflorus (Benth) has been shown to possess significant larvicidal activity against the mosquito Aedes aegypti. Bioassay-guided fractionation of the extract led to the isolation and characterization of the know diterpenoid furans 6alpha-hydroxyvouacapan-7beta,17beta-lactone (1), 6alpha,7beta-dihydroxyvouacapan- 17beta-oic acid (2) and methyl 6alpha,7beta-dihydroxyvouacapan-17beta-oate (3). The structures were established from infrared (IR), ultraviolet (UV), 1H-nuclear magnetic resonance (NMR), 13C-NMR, and mass spectral data: full NMR assignments are presented for compounds 1-3 and the diacetyl derivative of 3. Compounds 1-3 exhibited LC50 values of 50.08, 14.69, and 21.76 microg/mL against fourth-instar Aedes aegypti larvae.
已证明翼蕊豆（Pterodon polygalaeflorus (Benth)）种子的乙醇提取物对埃及伊蚊具有显著的杀幼虫活性。对该提取物进行生物测定导向的分级分离，导致分离并鉴定出已知的二萜呋喃类化合物6α-羟基沃阿卡潘-7β,17β-内酯（1）、6α,7β-二羟基沃阿卡潘-17β-酸（2）和6α,7β-二羟基沃阿卡潘-17β-甲酯（3）。通过红外（IR）、紫外（UV）、1H-核磁共振（NMR）、13C-NMR和质谱数据确定了结构：给出了化合物1-3以及3的二乙酰衍生物的完整NMR归属。化合物1-3对埃及伊蚊四龄幼虫的LC50值分别为50.08、14.69和21.76μg/mL。
