Bezerra-Silva Patrícia C, Santos Jefferson C, Santos Geanne K N, Dutra Kamilla A, Santana Andrea L B D, Maranhão Claudia A, Nascimento Márcia S, Navarro Daniela M A F, Bieber Lothar W
Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil; Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Exp Parasitol. 2015 Jun;153:160-4. doi: 10.1016/j.exppara.2015.03.018. Epub 2015 Mar 24.
The larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/mL. Fraction toluene/EtOAc (8:2) from this extract showed larvicidal activity (LC₅₀ = 34.90 ± 1.27 µg/mL). A mixture of two compounds identified as medicarpin and maackiain exhibited a very good larvicidal activity (sub-fraction 2, LC₅₀ = 17.5 ± 1.87 µg/mL) and maackiain showed to be a strong larvicidal compound (LC₅₀ = 21.95 ± 1.34 µg/mL). This result can be of value in the search for new natural larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.
按照世界卫生组织的指导方针，评估了三种硬木树种（Hymenaea stigonorcapa、Anadenanthera colubrina和Bowdichia virgilioides）提取物对埃及伊蚊四龄幼虫的杀幼虫活性。H. stignocarpa和A. colubrina的提取物表现出较弱的活性。Bowdichia virgilioides心材的环己烷提取物具有最高的杀幼虫效果，在浓度为50和100μg/mL时可导致100%的死亡率。该提取物的甲苯/乙酸乙酯（8:2）馏分表现出杀幼虫活性（LC₅₀ = 34.90 ± 1.27μg/mL）。鉴定为紫铆因和刺桐素的两种化合物的混合物表现出非常好的杀幼虫活性（亚馏分2，LC₅₀ = 17.5 ± 1.87μg/mL），刺桐素显示为一种强效杀幼虫化合物（LC₅₀ = 21.95 ± 1.34μg/mL）。这一结果对于从其他硬木植物提取物中寻找新的天然杀幼虫化合物具有参考价值，并且首次报道了Bowdichia virgilioides用于控制蚊虫媒介。
