Hu Bang-chuan, Chu Shao-li, Wang Ji-guang, Wang Zhi-hua, Wang Gu-liang, Gao Ping-jin, Zhu Ding-liang
Department of Hypertension, Ruijin Hospital, Shanghai Institute of Hypertension, Shanghai Jiaotong University Medical School, Shanghai 200025, China.
Zhonghua Yi Xue Za Zhi. 2006 Apr 25;86(16):1144-7.
To investigate the association of aldosterone synthase (CYP11B2) -344T/C polymorphism with plasma renin activity (PRA), and concentrations of angiotensin II (Ang II) and aldosterone (Aldo) in patients with essential hypertension (EH).
421 EH patients, 296 males and 125 females, aged 56.3 +/- 11.2, and 207 sex and aged-matched normotensive subjects were genotyped for the CYP11B2 -344T/C polymorphism by polymerase chain reaction-restriction fragment length polymorphism. PRA and plasma Ang II and Aldo concentrations were measured by radioimmunoassay among 328 EH patients in the supine position and 279 of the 328 subjects in the upright position.
No significant difference in genotype and allele frequencies of CYP11B2 -344T/C polymorphism was observed between the EH and normotensive subjects (P = 0.61 and P = 0.34 respectively). In the 328 EH patients undergoing measurement of PRA, Ang II, and Aldo, the plasma Aldo concentration of the TT subjects was 76.8 pg/ml, significantly higher than those of the TC subjects (58.8 pg/ml) and CC subjects (58.8 pg/ml, P = 0.02), after adjustment for age, sex and body mass index. No association was found between the -344T/C polymorphism and PRA and plasma Ang II in the supine position (P = 0.63 and P = 0.28 respectively). However, with similar adjustment applied, the plasma Ang II concentration in the upright position was higher in the CC subjects (85.7 pg/ml) than in the TC subjects (54.6 pg/ml) and TT subjects (51.8 pg/ml. P = 0.02).
In the EH patients, CYP11B2 -344T/C polymorphism is associated with plasma Aldo and the plasma Ang II concentration when the patients are in the upright position.
探讨醛固酮合成酶（CYP11B2）-344T/C基因多态性与原发性高血压（EH）患者血浆肾素活性（PRA）、血管紧张素II（Ang II）及醛固酮（Aldo）浓度的关系。
采用聚合酶链反应-限制性片段长度多态性方法，对421例EH患者（男296例，女125例，年龄56.3±11.2岁）及207例年龄、性别相匹配的血压正常者进行CYP11B2 -344T/C基因多态性分型。采用放射免疫分析法测定328例EH患者卧位及其中279例立位时的PRA、血浆Ang II及Aldo浓度。
EH患者与血压正常者之间CYP11B2 -344T/C基因多态性的基因型及等位基因频率无显著差异（分别为P = 0.61和P = 0.34）。在328例接受PRA、Ang II及Aldo测定的EH患者中，校正年龄、性别及体重指数后，TT基因型患者的血浆Aldo浓度为76.8 pg/ml，显著高于TC基因型患者（58.8 pg/ml）及CC基因型患者（58.8 pg/ml，P = 0.02）。卧位时，-344T/C基因多态性与PRA及血浆Ang II之间无相关性（分别为P = 0.63和P = 0.28）。然而，经过类似校正后，立位时CC基因型患者的血浆Ang II浓度（85.7 pg/ml）高于TC基因型患者（54.6 pg/ml）及TT基因型患者（51.8 pg/ml，P = 0.02）。
在EH患者中，CYP11B2 -344T/C基因多态性与立位时血浆Aldo及血浆Ang II浓度相关。
