一种普遍存在的植物酶——果胶甲基酯酶的新功能：RNA沉默增强子。
A novel function for a ubiquitous plant enzyme pectin methylesterase: the enhancer of RNA silencing.
作者信息
Dorokhov Yuri L, Frolova Olga Y, Skurat Eugene V, Ivanov Peter A, Gasanova Tatjana V, Sheveleva Anna A, Ravin Nikolay V, Mäkinen Kristiina M, Klimyuk Victor I, Skryabin Konstantin G, Gleba Yuri Y, Atabekov Joseph G
机构信息
Department of Virology and A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninsky Gory 1, Laboratory Building A, Moscow 119992, Russia.
出版信息
FEBS Lett. 2006 Jul 10;580(16):3872-8. doi: 10.1016/j.febslet.2006.06.013. Epub 2006 Jun 16.
Co-agroinjection of Nicotiana benthamiana leaves with the pectin methylesterase (proPME) gene and the TMV:GFP vector resulted in a stimulation of virus-induced RNA silencing (inhibition of GFP production, virus RNA degradation, stimulation of siRNAs production). Conversely, co-expression of TMV:GFP with either antisense PME construct or with enzymatically inactive proPME restored synthesis of viral RNA. Furthermore, expression of proPME enhanced the GFP transgene-induced gene silencing accompanied by relocation of the DCL1 protein from nucleus to the cytoplasm and activation of siRNAs and miRNAs production. It was hypothesized that DCL1 relocated to the cytoplasm may use as substrates both miRNA precursor and viral RNA. The capacity for enhancing the RNA silencing is a novel function for the polyfunctional PME.
将果胶甲酯酶（proPME）基因与烟草花叶病毒：绿色荧光蛋白（TMV:GFP）载体共同农杆菌注射本氏烟草叶片，会刺激病毒诱导的RNA沉默（抑制GFP产生、病毒RNA降解、刺激siRNA产生）。相反，TMV:GFP与反义PME构建体或无酶活性的proPME共表达可恢复病毒RNA的合成。此外，proPME的表达增强了GFP转基因诱导的基因沉默，同时伴随着DCL1蛋白从细胞核重新定位到细胞质以及siRNA和miRNA产生的激活。据推测，重新定位到细胞质中的DCL1可能将miRNA前体和病毒RNA都用作底物。增强RNA沉默的能力是多功能PME的一种新功能。
Zotero 插件