磺化聚（乙二醇）-丙烯酸酯共聚物通过替代途径激活补体。

Complement activation by sulfonated poly(ethylene glycol)-acrylate copolymers through alternative pathway.

作者信息

Jang Hong Seok, Ryu Kyu Eun, Ahn Woong Shick, Chun Heung Jae, Dal Park Hyung, Park Ki Dong, Kim Young Ha

机构信息

Department of Therapeutic Radiology, Uijeongbu St. Mary's Hospital, College of Medicine, Catholic University, Uijeongbu 480-130, Korea.

出版信息

Colloids Surf B Biointerfaces. 2006 Jul 1;50(2):141-6. doi: 10.1016/j.colsurfb.2006.03.024. Epub 2006 May 2.

DOI:10.1016/j.colsurfb.2006.03.024

PMID:16797170

Abstract

Previously, novel poly(ethylene glycol) (PEG) and sulfonated PEG acrylate (PEG-SO(3)A/OA) copolymers were prepared as coating and/or blending materials for biomedical applications. Surfaces modified with copolymers exhibited increased anti-coagulation properties and decreased plasma adsorption level due to increased hydrophilic properties and reorientation characteristics of PEG/PEG-SO(3)A chains in water phase. As continuation study, anti-complement effects of PEG-SO(3)/OA copolymers were investigated in vitro, and compared with those of low-density polyethylene (LDPE) and PEG/OA. C3 activation by PEG-SO(3)/OA samples was lower than that by PEG/OA samples, which was attributed to decreased surface nucleophile level of samples. PEG-SO(3)/OA samples increased inhibition of Bb production, resulting in decreased C5 activation. Owing to reduced activations of C3 and C5, PEG-SO(3)/OA samples markedly decreased SC5b-9 levels in plasma.

摘要

此前，新型聚乙二醇（PEG）和磺化聚乙二醇丙烯酸酯（PEG-SO(3)A/OA）共聚物被制备用作生物医学应用的涂层和/或共混材料。用共聚物修饰的表面由于水相中PEG/PEG-SO(3)A链的亲水性增加和重排特性，表现出增强的抗凝血性能和降低的血浆吸附水平。作为后续研究，对PEG-SO(3)/OA共聚物的抗补体作用进行了体外研究，并与低密度聚乙烯（LDPE）和PEG/OA的抗补体作用进行了比较。PEG-SO(3)/OA样品对C3的激活低于PEG/OA样品，这归因于样品表面亲核试剂水平的降低。PEG-SO(3)/OA样品增强了对Bb产生的抑制作用，导致C5激活减少。由于C3和C5的激活减少，PEG-SO(3)/OA样品显著降低了血浆中SC5b-9的水平。