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DNA quantification using EvaGreen and a real-time PCR instrument.

作者信息

Wang Weijie, Chen Kunsong, Xu Changjie

机构信息

State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, People's Republic of China.

出版信息

Anal Biochem. 2006 Sep 15;356(2):303-5. doi: 10.1016/j.ab.2006.05.027. Epub 2006 Jun 9.

DOI:10.1016/j.ab.2006.05.027
PMID:16797474
Abstract
摘要

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DNA quantification using EvaGreen and a real-time PCR instrument.使用EvaGreen和实时PCR仪器进行DNA定量。
Anal Biochem. 2006 Sep 15;356(2):303-5. doi: 10.1016/j.ab.2006.05.027. Epub 2006 Jun 9.
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Application of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplates.DNA特异性染料EvaGreen在微孔板中DNA常规定量分析中的应用。
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Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen.使用EvaGreen通过实时或定量PCR对人乳头瘤病毒16型E6癌基因序列进行定量分析。
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Real-time PCR fluorescent chemistries.实时聚合酶链反应荧光化学法
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Detection of PCR products in real time using light-up probes.使用点亮型探针实时检测聚合酶链式反应（PCR）产物。
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BEAMing up for detection and quantification of rare sequence variants.用于罕见序列变异检测和定量的BEAMing技术
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