The influence of subphase temperature on miltefosine-cholesterol mixed monolayers.

Effects of the subphase temperature on the surface pressure (pi)-area (A) isotherms of mixed monolayers of miltefosine (hexadecylphosphocholine), a potential anticancer drug, and cholesterol were investigated at the air/water interface, which were supplemented with Brewster angle microscopy (BAM) observations. Comparison of the collapse pressure values, mean molecular areas, excess areas and excess free energy of mixing between the mixed monolayer at various molar ratios and the pure component monolayers showed that, regardless of the subphase temperature, the investigated miltefosine-cholesterol system is much more stable than that the pure component monolayers, suggesting strong attractive interactions between miltefosine and cholesterol in mixed monolayers. As a consequence, it was postulated that stable "complexes" of the two components could form at the interface, for which stoichiometry may vary with the subphase temperature. Such "surface complexes" should be responsible for the contraction of the mean molecular area and thus the high stability of the mixed monolayer.