Zhu Qinglei, Qian Xiaoshun, Wang Shiwen, Yin Tong, Yang Jie, Xue Qiao, Xu Bin
Institute of Geriatric Cardiology, Chinese PLA General Hospital, Fuxing Road 28, Beijing 100853, China.
Exp Gerontol. 2006 Aug;41(8):771-7. doi: 10.1016/j.exger.2006.05.010. Epub 2006 Jun 22.
Multiple organ dysfunction syndrome (MODS) in the elderly is the most common cause of mortality in critically ill elderly patients, and it is different from MODS in the adult in clinic. Rare studies have been done on its pathogenesis and the comparison between adult and elderly MODS animal models. This work aimed at exploring the mechanisms mediating elderly MODS and compared this with adult MODS. Male Sprague-Dawley aged and adult rats were intraperitoneally injected with zymosan for incitement of MODS. Aged rats receiving zymosan showed severer pulmonary, cardiac and renal dysfunctions than adult rats. Likewise, the tissue lesions under light microscope in major organs of zymosan treated aged rats were much worse than those of zymosan treated adult rats. Moreover, zymosan treated aged rats showed 142% and 64% greater increase in pulmonary alveolar macrophages (AMs) apoptotic rate and serum TNF-alpha level, respectively, whereas 43% smaller increase in serum IL-10 level compared to zymosan treated adult rats. Furthermore, lung injury was much worse than that in other organs in zymosan treated aged rats. Overall, these results suggest that zymosan can be used in aged rats to incite MODS in the elderly. In the animal model of elderly MODS, there are (1) severer injury in lung, heart and kidney vs adult; (2) easier to develop severe systemic inflammatory response syndrome (SIRS) instead of compensatory anti-inflammatory response syndrome (CARS) compared to the adult; and (3) severer inflammation in lung than other organs indicative of the possible roles of lung in triggering MODS in the elderly.
老年多器官功能障碍综合征（MODS）是危重症老年患者最常见的死亡原因，且在临床上与成人MODS有所不同。关于其发病机制以及成人与老年MODS动物模型之间的比较，相关研究较少。本研究旨在探讨介导老年MODS的机制，并将其与成人MODS进行比较。将雄性老年和成年Sprague-Dawley大鼠腹腔注射酵母聚糖以诱发MODS。接受酵母聚糖注射的老年大鼠比成年大鼠表现出更严重的肺、心脏和肾脏功能障碍。同样，酵母聚糖处理的老年大鼠主要器官在光镜下的组织损伤比酵母聚糖处理的成年大鼠严重得多。此外，与酵母聚糖处理的成年大鼠相比，酵母聚糖处理的老年大鼠肺泡巨噬细胞（AMs）凋亡率和血清TNF-α水平分别升高142%和64%，而血清IL-10水平升高幅度小43%。此外，酵母聚糖处理的老年大鼠肺损伤比其他器官严重得多。总体而言，这些结果表明酵母聚糖可用于老年大鼠以诱发老年MODS。在老年MODS动物模型中，存在以下情况：（1）与成年相比，肺、心脏和肾脏损伤更严重；（2）与成年相比，更容易发生严重的全身炎症反应综合征（SIRS）而非代偿性抗炎反应综合征（CARS）；（3）肺炎症比其他器官更严重，表明肺在触发老年MODS中可能起作用。
