Obara Nobuko
Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
Methods Mol Biol. 2006;341:197-204. doi: 10.1385/1-59745-113-4:197.
Both cell-cell adhesion and secreted signaling molecules are involved in the regulation of normal tissue development and maintenance. beta-catenin seems to link cadherin-mediated cell-cell adhesion and Wnt signaling. Thus, its activity in particular cells or tissues, either during normal development or in tumorigenesis, has become a fascinating topic of investigation. Because the multiple functions of beta-catenin intimately relate to its amount and localization within a cell, immunohistochemical detection of this molecule simultaneously with the cell adhesion molecule cadherin should provide helpful information on its potential activities. This detection can be conducted on tissue sections by using appropriate tissue preparation procedures and primary antibodies from different species.
细胞间黏附与分泌的信号分子均参与正常组织的发育和维持调控。β-连环蛋白似乎在钙黏蛋白介导的细胞间黏附与Wnt信号传导之间起连接作用。因此，在正常发育过程中或肿瘤发生过程中，其在特定细胞或组织中的活性已成为一个引人入胜的研究课题。由于β-连环蛋白的多种功能与其在细胞内的含量和定位密切相关，因此与细胞黏附分子钙黏蛋白同时进行该分子的免疫组织化学检测，应能为其潜在活性提供有用信息。通过使用适当的组织制备程序和来自不同物种的一抗，可以在组织切片上进行这种检测。
