Wu Y Y, Yang X H
Department of Immunology, Chinese Academy of Medical Sciences, Beijing.
Proc Soc Exp Biol Med. 1991 Oct;198(1):620-4. doi: 10.3181/00379727-198-43298.
The effect of trace elements lithium, selenium, and zinc on interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC), MLA144, and Jurkat cell lines has been studied. Lithium markedly enhanced IL-2 production by MLA144 and PBMC, but not by Jurkat. Selenium could only enhance IL-2 production by MLA144, whereas in none of these three systems was IL-2 production altered by zinc. The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. The effect of lithium on IL-2 production was in the early phase of lymphocyte activation. The addition of cholera toxin or theophylline to phytohemagglutinin-stimulated PBMC culture suppressed IL-2 production. However, IL-2 production could be restored by lithium. There was a corresponding increase in cAMP in cholera toxin or theophylline-treated PBMC, which could be reversed by lithium. Therefore, lithium restores IL-2 production via a decrease in cAMP.
研究了微量元素锂、硒和锌对外周血单个核细胞（PBMC）、MLA144和Jurkat细胞系产生白细胞介素2（IL-2）的影响。锂显著增强了MLA144和PBMC产生IL-2的能力，但对Jurkat细胞系无此作用。硒仅能增强MLA144产生IL-2的能力，而在这三个系统中，锌均未改变IL-2的产生。锂对IL-2产生的增强作用在以下方面与十四酰佛波醇乙酸酯（TPA）有所不同：（i）TPA可逆转抗CD2单克隆抗体对IL-2产生的抑制作用，而锂不能；（ii）锂不能像TPA那样与抗CD3单克隆抗体协同诱导IL-2的产生。锂对IL-2产生的影响发生在淋巴细胞激活的早期阶段。在植物血凝素刺激的PBMC培养物中添加霍乱毒素或茶碱会抑制IL-2的产生。然而，锂可恢复IL-2的产生。在霍乱毒素或茶碱处理的PBMC中，cAMP相应增加，而锂可使其逆转。因此，锂通过降低cAMP来恢复IL-2的产生。
