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来自海洋细菌海云台副球菌的虾青素生物合成基因的功能表达。

Functional expression of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis.

作者信息

Lee Jae Hyung, Kim Young Tae

机构信息

Department of Microbiology, Pukyong National University, Busan, 608-737, South Korea.

出版信息

Biotechnol Lett. 2006 Aug;28(15):1167-73. doi: 10.1007/s10529-006-9072-0. Epub 2006 Jun 24.

DOI:10.1007/s10529-006-9072-0
PMID:16799760
Abstract

The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product has been purified to homogeneity. Their molecular characteristics, including enzymatic activities, are reported here.

摘要

海州副球菌中的虾青素生物合成基因簇由六个基因组成

crtW、crtZ、crtY、crtI、crtB和crtE,分别包含726、486、1158、1503、912和879个碱基对。海州副球菌的各个类胡萝卜素生物合成基因现已在大肠杆菌中表达,并且每个基因产物都已纯化至同质。本文报道了它们的分子特征,包括酶活性。

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