Cheng Shiwei, Wei Dongzhi, Song Qingxun, Zhao Xiangguo
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, PR China.
Biotechnol Lett. 2006 Jul;28(14):1129-33. doi: 10.1007/s10529-006-9067-x. Epub 2006 Jun 24.
The activity of penicillin G acylase from Alcaligenes faecalis increased 7.5-fold when cells were permeabilized with 0.3% (w/v) CTAB. The treated cells were entrapped by polyvinyl alcohol crosslinked with boric acid, and crosslinked with 2% (v/v) glutaraldehyde to increase the stability. The conversion yield of penicillin G to 6-aminopenicillanic acid was 75% by immobilized system in batch reaction. No activity was lost after 15 cycles and about 65% enzyme activity was retained at the end of the 31th cycle.
用0.3%（w/v）十六烷基三甲基溴化铵使粪产碱杆菌的青霉素G酰化酶细胞透性化后，其活性提高了7.5倍。经处理的细胞被用硼酸交联的聚乙烯醇包埋，并用2%（v/v）戊二醛交联以提高稳定性。在分批反应中，固定化体系将青霉素G转化为6-氨基青霉烷酸的转化率为75%。15个循环后活性没有损失，在第31个循环结束时仍保留约65%的酶活性。
