Tzotzes Vassilios, Tzilalis Vassilios, Giannakakis Sotirios, Saranteas Theodosios, Papas Asimakis, Mourouzis Iordanis, Mourouzis Constantinos, Zarros Apostolos, Pantos Constantinos, Cokkinos Dennis, Carageorgiou Haris
Department of Experimental Pharmacology, Medical School, University of Athens, 75 Mikras Asias str., Goudi, GR-11527, Athens, Greece.
Biometals. 2007 Feb;20(1):83-91. doi: 10.1007/s10534-006-9017-z. Epub 2006 Jun 27.
The effect of acute and chronic cadmium (Cd) administration on the vascular function of the rat aorta was studied. The rats were randomly divided into four main groups (A: saline controls under chronic administration, B: Cd-treated rats under chronic administration, C: saline controls under acute administration, D: Cd-treated rats under acute administration). After their sacrifice, the aortic rings were divided into rings with endothelium (E+) and without (E-), and suspended in an isolated organ bath with Krebs - Henseleit buffer. Maximal tension (T max, in g) was measured in response to potassium chloride (KCl) and phenylephrine (PE) in all aortic rings. Relaxation response to acetylcholine (ACh) administration was expressed as percent of maximal tension induced by PE. Chronic administration: A statistically significant increase of the contraction was observed between groups B (i.m. Cd 0.5 mg/kg for 120 days) and A (i.m. 0.9% NaCl for 120 days) in response to KCl (20-60 mM) and the T max as well (in both the E+ and the E- subgroups). No statistically significant difference was observed in response to PE and ACh exposure. Acute administration: A statistically significant increase was observed between group D(E+) (i.m. Cd 2 mg/kg, 8 h before sacrifice) and group C(E+) (i.m. 0.9% NaCl, 8 h before sacrifice) in response to 10-30 mM of KCl, and a significant decrease between D(E-) and C(E-) in response to 10(-7)-10(-6) M of PE, though T (max) was increased between D(E-) and C(E-) with PE exposure. The contractile response levels of the E+ aortic rings to PE and ACh showed no statistically significant difference.
研究了急性和慢性镉（Cd）给药对大鼠主动脉血管功能的影响。大鼠被随机分为四个主要组（A：慢性给药下的生理盐水对照组，B：慢性给药下的镉处理大鼠，C：急性给药下的生理盐水对照组，D：急性给药下的镉处理大鼠）。处死大鼠后，将主动脉环分为有内皮（E+）和无内皮（E-）的环，并悬浮于含有Krebs - Henseleit缓冲液的离体器官浴中。测量所有主动脉环对氯化钾（KCl）和去氧肾上腺素（PE）的最大张力（T max，单位为g）。乙酰胆碱（ACh）给药后的舒张反应以PE诱导的最大张力的百分比表示。慢性给药：在B组（肌肉注射Cd 0.5 mg/kg，持续120天）和A组（肌肉注射0.9%氯化钠，持续120天）之间，观察到对KCl（20 - 60 mM）以及T max的收缩反应有统计学显著增加（在E+和E-亚组中均如此）。在对PE和ACh暴露的反应中未观察到统计学显著差异。急性给药：在D组（E+）（肌肉注射Cd 2 mg/kg，处死前8小时）和C组（E+）（肌肉注射0.9%氯化钠，处死前8小时）之间，观察到对10 - 30 mM KCl的反应有统计学显著增加，并且在D组（E-）和C组（E-）之间，对10(-7)-10(-6) M PE的反应有显著降低，尽管在PE暴露下D组（E-）和C组（E-）之间T（max）增加。E+主动脉环对PE和ACh的收缩反应水平无统计学显著差异。
