Qutob S S, Chauhan V, Bellier P V, Yauk C L, Douglas G R, Berndt L, Williams A, Gajda G B, Lemay E, Thansandote A, McNamee J P
Consumer and Clinical Radiation Protection Bureau, Healthy Environment and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada, K1A 1C1.
Radiat Res. 2006 Jun;165(6):636-44. doi: 10.1667/RR3561.1.
The widespread use of mobile phones has led to public concerns about the health effects associated with exposure to radiofrequency (RF) fields. The paramount concern of most persons relates to the potential of these fields to cause cancer. Unlike ionizing radiation, RF fields used for mobile telecommunications (800-1900 MHz) do not possess sufficient energy to directly damage DNA. Most rodent bioassay and in vitro genotoxicity/mutation studies have reported that RF fields at non-thermal levels have no direct mutagenic, genotoxic or carcinogenic effects. However, some evidence has suggested that RF fields may cause detectable postexposure changes in gene expression. Therefore, the purpose of this study was to assess the ability of exposure to a 1.9 GHz pulse-modulated RF field for 4 h at specific absorption rates (SARs) of 0.1, 1.0 and 10.0 W/kg to affect global gene expression in U87MG glioblastoma cells. We found no evidence that non-thermal RF fields can affect gene expression in cultured U87MG cells relative to the nonirradiated control groups, whereas exposure to heat shock at 43 degrees C for 1 h up-regulated a number of typical stress-responsive genes in the positive control group. Future studies will assess the effect of RF fields on other cell lines and on gene expression in the mouse brain after in vivo exposure.
手机的广泛使用引发了公众对接触射频（RF）场相关健康影响的担忧。大多数人最主要的担忧是这些场可能致癌。与电离辐射不同，用于移动电信的射频场（800 - 1900兆赫）没有足够的能量直接损伤DNA。大多数啮齿动物生物测定和体外遗传毒性/突变研究报告称，非热水平的射频场没有直接的诱变、遗传毒性或致癌作用。然而，一些证据表明射频场可能会在暴露后引起可检测到的基因表达变化。因此，本研究的目的是评估在比吸收率（SARs）为0.1、1.0和10.0瓦/千克的条件下，暴露于1.9吉赫脉冲调制射频场4小时对U87MG胶质母细胞瘤细胞中全球基因表达的影响。我们没有发现证据表明相对于未辐照的对照组，非热射频场会影响培养的U87MG细胞中的基因表达，而在阳性对照组中，43摄氏度热休克1小时会上调一些典型的应激反应基因。未来的研究将评估射频场对其他细胞系以及体内暴露后对小鼠大脑基因表达的影响。
