Srivatsan E S, Misra B C, Venugopalan M, Wilczynski S P
Division of Hematology/Oncology, Children's Hospital, Los Angeles, CA 90027.
Am J Hum Genet. 1991 Oct;49(4):868-77.
The HeLa cell (a cervical carcinoma cell line) tumor-suppressor gene has been localized to the long arm of chromosome 11 by molecular genetic studies of nontumorigenic and tumorigenic hybrids derived from normal chromosome 11 x HeLa cell fusions. In the present study, 33 primary cervical carcinoma samples were analyzed using chromosome 11-specific polymorphic DNA markers. The RFLP analysis indicated a somatic loss of chromosome 11 heterozygosity in 10 (30%) of the primary tumors. Preferential loss of the long arm of the chromosome was observed in two of the primary tumors. In addition, at least eight-fold amplification of sequences in the q13 region, including those coding for the fibroblast growth factor-related gene (int-2), was observed in one of the primary tumors. These results suggest a possible role for gene(s) localized to chromosome 11, possibly that localized to the long arm in the development and/or progression of cervical carcinomas.
通过对源自正常11号染色体与海拉细胞融合的非致瘤性和致瘤性杂种进行分子遗传学研究，已将海拉细胞（一种宫颈癌细胞系）的肿瘤抑制基因定位到11号染色体的长臂上。在本研究中，使用11号染色体特异性多态性DNA标记对33份原发性宫颈癌样本进行了分析。限制性片段长度多态性（RFLP）分析表明，10例（30%）原发性肿瘤中存在11号染色体杂合性的体细胞缺失。在其中两例原发性肿瘤中观察到该染色体长臂的优先缺失。此外，在其中一例原发性肿瘤中，观察到q13区域的序列至少扩增了八倍，包括那些编码成纤维细胞生长因子相关基因（int-2）的序列。这些结果表明，定位于11号染色体上的基因，可能是定位于长臂上的基因，在宫颈癌的发生和/或发展中可能发挥作用。
