Jackson L E, Faris B, Martin B M, Jones H V, Rich C B, Foster J A, Franzblau C
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Biochem Biophys Res Commun. 1991 Sep 16;179(2):939-44. doi: 10.1016/0006-291x(91)91909-v.
When beta-aminopropionitrile (BAPN) is added to neonatal rat aortic smooth muscle cell cultures there is a decrease in insoluble elastin accumulation with a concomitant increase in tropoelastin and tropoelastin fragments in the culture medium. The experiments described here examine the biological significance of this fragmentation. BAPN, as well as purified tropoelastin fragments isolated from spent medium of cells grown in the presence of BAPN, were added to cultures. A decrease in elastin mRNA was observed in cultures grown in the presence of BAPN and also in those cultures to which the purified tropoelastin moieties were added. These studies indicate that the inhibition of lysyl oxidase by BAPN prevents elastin crosslinking which results in an increase in tropoelastin moieties, thus leading to a down regulation of the steady state levels of elastin mRNA.
当向新生大鼠主动脉平滑肌细胞培养物中添加β-氨基丙腈（BAPN）时，不溶性弹性蛋白的积累会减少，同时培养基中的原弹性蛋白和原弹性蛋白片段会增加。此处描述的实验研究了这种片段化的生物学意义。将BAPN以及从在BAPN存在下生长的细胞的用过的培养基中分离出的纯化的原弹性蛋白片段添加到培养物中。在存在BAPN的培养物以及添加了纯化的原弹性蛋白部分的培养物中均观察到弹性蛋白mRNA减少。这些研究表明，BAPN对赖氨酰氧化酶的抑制作用会阻止弹性蛋白交联，从而导致原弹性蛋白部分增加，进而导致弹性蛋白mRNA稳态水平的下调。
