Albuquerque Joaquim F S, Ferra Maria A, Portela-Gomes Guida M
Department of Surgery 2, Santa Maria University Hospital, Lisbon, Portugal.
Scand J Gastroenterol. 2006 Aug;41(8):963-8. doi: 10.1080/00365520500527581.
Colectomized patients often have diarrhoea and increased gastric acid secretion. Although serotonin influences gastrointestinal (GI) motility and secretion, GI serotonin-producing enterochromaffin (EC) cells have not been investigated after colectomy, nor have the antral gastrin cells. The aim of this experimental study was to investigate the GI tract in rats 8 weeks after subtotal colectomy, with particular emphasis on the frequency and distribution of EC and gastrin cells.
Immunohistochemical techniques were used to identify the two endocrine cell types.
The colectomized animals had diarrhoea. Body-weight was lower and the small intestine shorter in the colectomized animals compared with sham-operated and untreated controls. In the two surgically treated groups, the antral mucosa was thinner and the small intestinal mucosa was thicker compared with that of the untreated rats, whereas the thickness of the rectum of the colectomized rats was increased compared with that of the control groups. In the colectomized animals, the number of EC cells was increased in the small intestine and rectum, whereas the numbers of both EC and gastrin cells were decreased in the antrum.
The results indicate that colectomy exerts a significant influence on the GI mucosa and on the endocrine cell systems studied. An increased number of EC cells can result in alterations in motility and secretion, which may be important in the pathogenesis of the diarrhoea that often occurs after colectomy.
结肠切除术后患者常出现腹泻且胃酸分泌增加。尽管血清素会影响胃肠（GI）蠕动和分泌，但结肠切除术后尚未对产生血清素的胃肠肠嗜铬（EC）细胞进行研究，胃窦促胃液素细胞也未被研究。本实验研究的目的是调查大鼠结肠次全切除术后8周时的胃肠道情况，特别关注EC细胞和促胃液素细胞的频率及分布。
采用免疫组织化学技术鉴定这两种内分泌细胞类型。
结肠切除术后的动物出现腹泻。与假手术组和未处理的对照组相比，结肠切除术后的动物体重更低，小肠更短。在两个手术治疗组中，与未处理的大鼠相比，胃窦黏膜更薄，小肠黏膜更厚，而结肠切除术后大鼠的直肠厚度相较于对照组有所增加。在结肠切除术后的动物中，小肠和直肠中的EC细胞数量增加，而胃窦中EC细胞和促胃液素细胞的数量均减少。
结果表明结肠切除术对胃肠道黏膜及所研究内分泌细胞系统有显著影响。EC细胞数量增加可导致蠕动和分泌改变，这可能在结肠切除术后常出现的腹泻发病机制中起重要作用。
