Hermans Armand P H M, Beuling Annelien M, van Hoek Angela H A M, Aarts Henk J M, Abee Tjakko, Zwietering Marcel H
Laboratory of Food Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, PO Box 8129, 6700 EV Wageningen, The Netherlands.
RIKILT Institute of Food Safety, PO Box 230, 6700 AE Wageningen, The Netherlands.
Microbiology (Reading). 2006 Jul;152(Pt 7):2137-2147. doi: 10.1099/mic.0.28850-0.
Recently, the authors identified Salmonella enterica serovar Typhimurium (S. Typhimurium) definitive type (DT)104-specific sequences of mainly prophage origin by genomic subtractive hybridization. In the present study, the distribution of the prophages identified, ST104 and ST64B, and the novel prophage remnant designated prophage ST104B, was tested among 23 non-DT104 S. Typhimurium isolates of different phage types and 19 isolates of the DT104 subtypes DT104A, DT104B low and DT104L, and the DT104-related type U302. The four S. Typhimurium prophages Gifsy-1, Gifsy-2, Fels-1 and Fels-2 were also included. Analysis of prophage distribution in different S. Typhimurium isolates may supply additional information to enable development of a molecular method as an alternative to phage typing. Furthermore, the presence of the common DT104 antibiotic resistance genes for the penta-resistance type ACSSuT, aadA2, floR, pse-1, sul1 and tet(G), was also studied because of the authors' focus on this emerging type. Based on differences in prophage presence within their genome, it was possible to divide S. Typhimurium isolates into 12 groups. Although no clear relationship was found between different phage type and prophage presence, discrimination could be made between the different DT104 subtypes based on diversity in the presence of prophages ST104, ST104B and ST64B. The novel prophage remnant ST104B, which harbours a homologue of the Escherichia coli O157 : H7 HldD LPS assembly-related protein, was identified only in the 14 DT104L isolates and in the DT104-related U302 isolate. In conclusion, the presence of the genes for penta-resistance type ACSSuT, the HldD homologue containing ST104 prophage remnant and phage type DT104L are most likely common features of the emerging subtype of S. Typhimurium DT104.
最近，作者通过基因组消减杂交鉴定出肠炎沙门氏菌鼠伤寒血清型（鼠伤寒沙门氏菌）确定型（DT）104特异性序列，其主要来源于前噬菌体。在本研究中，对所鉴定的前噬菌体ST104和ST64B以及新命名的前噬菌体残余物前噬菌体ST104B在23株不同噬菌体类型的非DT104鼠伤寒沙门氏菌分离株、19株DT104亚型DT104A、低毒力DT104B和DT104L以及与DT104相关的U302型菌株中的分布情况进行了检测。还纳入了4种鼠伤寒沙门氏菌前噬菌体Gifsy-1、Gifsy-2、Fels-1和Fels-2。分析不同鼠伤寒沙门氏菌分离株中的前噬菌体分布可能会提供更多信息，以便开发一种分子方法作为噬菌体分型的替代方法。此外，由于作者关注这种新出现的类型，因此还研究了五重耐药型ACSSuT、aadA2、floR、pse-1、sul1和tet(G)等常见DT104抗生素抗性基因的存在情况。基于其基因组中前噬菌体存在情况的差异，有可能将鼠伤寒沙门氏菌分离株分为12组。虽然未发现不同噬菌体类型与前噬菌体存在之间有明确关系，但根据前噬菌体ST104、ST104B和ST64B存在情况的差异，可以区分不同的DT104亚型。仅在14株DT1所鉴定的前噬菌体ST104和ST64B以及新命名的前噬菌体残余物前噬菌体ST104B在23株不同噬菌体类型的非DT104鼠伤寒沙门氏菌分离株、19株DT104亚型DT104A、低毒力DT104B和DT104L以及与DT104相关的U302型菌株中的分布情况进行了检测。还纳入了4种鼠伤寒沙门氏菌前噬菌体Gifsy-1、Gifsy-2、Fels-1和Fels-2。分析不同鼠伤寒沙门氏菌分离株中的前噬菌体分布可能会提供更多信息，以便开发一种分子方法作为噬菌体分型的替代方法。此外，由于作者关注这种新出现的类型，因此还研究了五重耐药型ACSSuT、aadA2、floR、pse-1、sul1和tet(G)等常见DT104抗生素抗性基因的存在情况。基于其基因组中前噬菌体存在情况的差异，有可能将鼠伤寒沙门氏菌分离株分为12组。虽然未发现不同噬菌体类型与前噬菌体存在之间有明确关系，但根据前噬菌体ST104、ST104B和ST64B存在情况的差异，可以区分不同的DT104亚型。仅在14株DT104L分离株和DT104相关的U302分离株中鉴定出了新的前噬菌体残余物ST104B，它含有大肠杆菌O157:H7 HldD脂多糖组装相关蛋白的同源物。总之，五重耐药型ACSSuT基因、含有ST104前噬菌体残余物的HldD同源物以及噬菌体类型DT104L的存在很可能是鼠伤寒沙门氏菌DT104新出现亚型的共同特征。 4L分离株和DT104相关的U302分离株中鉴定出了新的前噬菌体残余物ST104B，它含有大肠杆菌O157:H7 HldD脂多糖组装相关蛋白的同源物。总之，五重耐药型ACSSuT基因、含有ST104前噬菌体残余物的HldD同源物以及噬菌体类型DT104L的存在很可能是鼠伤寒沙门氏菌DT104新出现亚型的共同特征。
