Zanjani E D, Mackintosh F R, Harrison M R
Department of Medicine, Veterans Affairs Medical Center, Reno, NV 89520.
Blood Cells. 1991;17(2):349-63; discussion 364-6.
Bone marrow transplantation to reconstitute defective hematopoietic cell lines in children with congenital defects is limited by donor availability, graft rejection, and graft-versus-host disease (GVHD). These problems can be eliminated by transplanting normal preimmune fetal hematopoietic stem cells (HSC) into an unrelated preimmune fetal recipient. We show here that injections of allogeneic fetal stem cells into preimmune fetal lambs and monkeys result in long-term stable hematopoietic chimerism. HSCs harvested from the livers of preimmune fetal sheep and monkeys when injected into the peritoneal cavity of young unrelated fetal sheep and monkey recipients results in stable, long-term postnatal hematopoietic chimerism involving lymphoid, erythroid, and myeloid cells of donor origin. Donor cell engraftment was achieved without the use of cytoablative procedures and without the development of GVHD.
对于患有先天性缺陷的儿童，通过骨髓移植来重建有缺陷的造血细胞系，会受到供体可用性、移植物排斥和移植物抗宿主病（GVHD）的限制。将正常的免疫前胎儿造血干细胞（HSC）移植到不相关的免疫前胎儿受体中，可以消除这些问题。我们在此表明，将同种异体胎儿干细胞注射到免疫前的胎儿羔羊和猴子体内，会导致长期稳定的造血嵌合体。从免疫前胎儿绵羊和猴子的肝脏中采集的造血干细胞，当注射到年轻的不相关胎儿绵羊和猴子受体的腹腔中时，会导致稳定的、长期的出生后造血嵌合体，其中包括供体来源的淋巴细胞、红细胞和髓细胞。在不使用细胞消融程序且不发生移植物抗宿主病的情况下，实现了供体细胞的植入。
