Tateya Tomoko, Tateya Ichiro, Bless Diane M
Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ann Otol Rhinol Laryngol. 2006 Jun;115(6):469-76. doi: 10.1177/000348940611500612.
The collagen subtypes in human vocal folds are of particular interest, because each collagen subtype has different features that make it uniquely suited for performing specific tissue tasks and each collagen subtype can affect the tissue properties of the vocal fold lamina propria.
Human vocal folds from 5 autopsy cases (less than 65 years old) were examined by immunohistochemistry for collagen types I, III, IV, and V and elastin.
Collagen type III was distributed throughout the whole lamina propria. Type I was found just beneath the basal membrane, in the deep layer of the lamina propria and in the anterior and posterior maculae flavae. Types IV and V were present in the epithelial and endothelial basal membrane. Three-dimensional images from thick specimens reconstructed with confocal microscopy showed 2 distinct patterns: type III fibers were wavy, collagenous fibers, as previously observed in the vocal folds, and type I fibers were thinner than type III fibers. These results suggest that type III fibers help maintain the lamina propria structure and that type I fibers provide the tensile strength required around the basal membrane and vocal ligament to maintain the vocal fold shape while withstanding vibratory forces.
人类声带中的胶原亚型特别引人关注，因为每种胶原亚型都具有不同特性，使其特别适合执行特定的组织任务，并且每种胶原亚型都会影响声带固有层的组织特性。
对5例（年龄小于65岁）尸检病例的人类声带进行免疫组织化学检查，以检测I型、III型、IV型和V型胶原以及弹性蛋白。
III型胶原分布于整个固有层。I型胶原位于基底膜下方、固有层深层以及前后黄韧带斑。IV型和V型胶原存在于上皮和内皮基底膜中。用共聚焦显微镜重建的厚标本三维图像显示出两种不同模式：III型纤维呈波浪状胶原纤维，如先前在声带中观察到的那样，I型纤维比III型纤维细。这些结果表明，III型纤维有助于维持固有层结构，而I型纤维在基底膜和声带韧带周围提供维持声带形状并承受振动力量所需的拉伸强度。
