Malik R, Gope M, Womer R B, Nagashunmugam T, Margolin J F, Basu A, Scher C D
Division of Oncology, Children's Hospital of Philadelphia, Pennsylvania 19104.
Cancer Res. 1991 Oct 15;51(20):5626-31.
Southern digests of DNA from 15 human connective tissue tumor-derived cell lines (and cell strains) and from peripheral blood lymphocytes from normal volunteers were prepared to compare structure of the beta-platelet-derived growth factor receptor gene in normal and tumor-derived cells. The tumor-derived samples had no alterations in gene structure, nor was gene amplification evident. Two restriction fragment length polymorphisms were detected within the beta-receptor gene. Expression of the alpha- and beta-platelet-derived growth factor receptor was quantified using Northern blots. Expression was not tumor-type specific. For example, one rhabdomyosarcoma cell line expressed only the alpha-receptor, whereas two others expressed the beta. In contrast, a human fibroblast cell strain expressed both receptor types. Alterations in receptor expression may be a result of the tumorigenic process.
制备了来自15个人类结缔组织肿瘤衍生细胞系（和细胞株）以及正常志愿者外周血淋巴细胞的DNA的Southern印迹，以比较正常细胞和肿瘤衍生细胞中β-血小板衍生生长因子受体基因的结构。肿瘤衍生样本的基因结构没有改变，基因扩增也不明显。在β-受体基因内检测到两个限制性片段长度多态性。使用Northern印迹定量α-和β-血小板衍生生长因子受体的表达。表达不是肿瘤类型特异性的。例如，一个横纹肌肉瘤细胞系仅表达α-受体，而另外两个则表达β-受体。相比之下，一个人成纤维细胞株表达两种受体类型。受体表达的改变可能是致瘤过程的结果。
