Wang Qi-ming, Li Xiao, Jia Lian-qun, Yang Kai-ming, Zhou Hong-ying, Yang Hui-jun
Department of Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Jul;22(4):454-7.
To investigate the role of Wnt/beta-catenin signaling transduction pathway in rat hepatocarcinogenesis.
The mRNAs of Wnt1, beta-catenin, APC, cyclin D1 and c-myc genes were amplified by using of semiquantitative reverse transcription polymerase chain reaction (RT-PCR) from normal rat livers, atypical hyperplasia livers and hepatoma tissues, respectively. Then the proteins expression of beta-catenin, APC and cyclin D1 was examined by immunohistochemical staining.
In normal rat livers, the mRNAs of Wnt1, cyclin D1 and c-myc genes were not detected and only beta-catenin protein was observed to have low expression at cellular membrane. However, 14 weeks after cancer induction in atypical hyperplasia livers, beta-catenin protein and APC protein were accumulated in cytoplasm. Meanwhile, cyclin D1 protein was detected in cytoplasm and/or nucleus in some cells. 16 weeks after cancer induction in hepatoma tissues, the mRNAs and protein expression of beta-catenin, APC, cyclin D1 and c-myc genes were detected except Wnt1.
The activation of Wnt/beta-catenin signaling transduction pathway might be one of the reasons for rat hepatocarcinogenesis.
探讨Wnt/β-连环蛋白信号转导通路在大鼠肝癌发生中的作用。
分别采用半定量逆转录聚合酶链反应（RT-PCR）从正常大鼠肝脏、非典型增生肝脏和肝癌组织中扩增Wnt1、β-连环蛋白、APC、细胞周期蛋白D1和c-myc基因的mRNA。然后通过免疫组织化学染色检测β-连环蛋白、APC和细胞周期蛋白D1的蛋白表达。
在正常大鼠肝脏中，未检测到Wnt1、细胞周期蛋白D1和c-myc基因的mRNA，仅观察到β-连环蛋白在细胞膜上有低表达。然而，在非典型增生肝脏致癌诱导14周后，β-连环蛋白和APC蛋白在细胞质中积累。同时，在一些细胞的细胞质和/或细胞核中检测到细胞周期蛋白D1蛋白。在肝癌组织致癌诱导16周后，除Wnt1外，检测到β-连环蛋白、APC、细胞周期蛋白D1和c-myc基因的mRNA和蛋白表达。
Wnt/β-连环蛋白信号转导通路的激活可能是大鼠肝癌发生的原因之一。
