Maina Naomi W N, Kunz Christina, Brun Reto
Trypanosomiasis Research Institute (TRC), P.O. Box 362, Kikuyu, Kenya.
Acta Trop. 2006 Jul;98(3):207-11. doi: 10.1016/j.actatropica.2005.11.011. Epub 2006 Jun 27.
There have been major advances in the formulation of cryomedia for spermatozoa owing to their economic importance. In this study, the suitability of the commercial cryomedium Triladyl developed for bull semen was evaluated for the cryopreservation of Trypanosoma brucei gambiense. Cryopreservation efficacy was determined by direct counting of motile trypanosomes and by viability assessment using in vitro and in vivo methods. Culture medium containing 10% glycerol was used as the control. Trypanosomes cryopreserved in Triladyl demonstrated a higher in vitro viability than those in culture medium with 10% glycerol. Similar results were obtained in vivo in immunosuppressed Mastomys natalensis. Trypanosomes cryopreserved in Triladyl showed better growth characteristics than those in culture medium with glycerol. It can be concluded that the use of Triladyl in the cryopreservation of T. b. gambiense leads to a better survival of the trypanosomes which could lead to an improved isolation of T. b. gambiense from sleeping sickness patients.
由于精子在经济方面的重要性，精子冷冻保存液的配方取得了重大进展。在本研究中，评估了专为牛精液开发的商业冷冻保存液Triladyl对布氏冈比亚锥虫冷冻保存的适用性。通过直接计数活动锥虫以及使用体外和体内方法进行活力评估来确定冷冻保存效果。含有10%甘油的培养基用作对照。在Triladyl中冷冻保存的锥虫在体外的活力高于在含10%甘油的培养基中的锥虫。在免疫抑制的南非乳鼠体内也获得了类似结果。在Triladyl中冷冻保存的锥虫比在含甘油的培养基中的锥虫表现出更好的生长特性。可以得出结论，在布氏冈比亚锥虫的冷冻保存中使用Triladyl可使锥虫更好地存活，这可能有助于从昏睡病患者中更好地分离出布氏冈比亚锥虫。
