Zhao Yulian, Zhou Liang, Liu Bisheng, Deng Yun, Wang Ying, Wang Yuequn, Huang Wen, Yuan Wuzhou, Wang Zequn, Zhu Chuanbing, Liu Mingyao, Wu Xiushan, Li Yongqing
The Center for Heart Development, Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People's Republic of China.
Biochem Biophys Res Commun. 2006 Aug 11;346(4):1191-9. doi: 10.1016/j.bbrc.2006.06.031. Epub 2006 Jun 15.
Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of eukaryotic cell regulation. The zinc-finger-containing transcription factors have been previously revealed to be involved in the regulation of the MAPK signaling pathways. Here, we have identified a novel human zinc-finger transcriptional repressor, ZNF325, that contains a RBaK-like RB-binding domain and 15 tandem repeated C2H2 type zinc fingers. Northern blot analysis indicates that a 2.7 kb transcript specific for ZNF325 is widely expressed in all tissues examined at adult stage and in most of the embryonic tissues. Overexpression of ZNF325 in COS-7 cells inhibits the transcriptional activities of AP-1 and SRE. The deletion and RNAi analysis indicate that the C2H2 zinc finger motifs represent the basal transcriptional repressive activity. These results indicate that the ZNF325 protein may act as a novel transcription repressor in MAPK signaling pathway to mediate cellular functions.
丝裂原活化蛋白激酶（MAPK）信号转导通路是真核细胞调控中最广泛存在的机制之一。先前已揭示含锌指的转录因子参与MAPK信号通路的调控。在此，我们鉴定出一种新型人类锌指转录抑制因子ZNF325，它含有一个类RBaK的RB结合结构域和15个串联重复的C2H2型锌指。Northern印迹分析表明，ZNF325特异的2.7 kb转录本在成年阶段检测的所有组织以及大多数胚胎组织中广泛表达。ZNF325在COS-7细胞中的过表达抑制了AP-1和SRE的转录活性。缺失和RNA干扰分析表明，C2H2锌指基序代表基础转录抑制活性。这些结果表明，ZNF325蛋白可能作为MAPK信号通路中的一种新型转录抑制因子来介导细胞功能。
