Hall Jean A, Picton Rebecca A, Finneran Phyllis S, Bird Karyn E, Skinner Monica M, Jewell Dennis E, Zicker Steven
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Vet Immunol Immunopathol. 2006 Sep 15;113(1-2):224-33. doi: 10.1016/j.vetimm.2006.03.019. Epub 2006 Jun 27.
The study objective was to determine the effects of feeding food enriched in antioxidants and a program of environmental/cognitive enrichment on selected ex vivo assays of inflammatory and immune cells in healthy geriatric Beagle dogs (n=21). Four groups of dogs were tested using a 2 x 2 factorial design. The 2-year longitudinal study included both nutritional (control food or antioxidant-fortified food) and behavioral (normal level or cognitive enrichment) interventions. Behavior enrichment included increased exercise, environmental enrichment, and a series of learning tasks. Phagocytosis of opsonized latex-coated beads by peripheral blood neutrophils was measured by flow cytometry and found to be significantly increased in dogs receiving both dietary antioxidants and cognitive enrichment. Simultaneous stimulation of cells with Con A and suppression with Dex resulted in decreased lymphocyte proliferation in dogs receiving both dietary antioxidants and cognitive enrichment, compared to dogs receiving dietary antioxidants or cognitive enrichment alone. There were no significant differences between the groups of dogs for percentages of CD4 and CD8 T-lymphocyte subpopulations before or after lymphocyte stimulation with Con A. These results support our hypothesis that both dietary antioxidants and behavioral enrichment enhance host defense mechanisms.
本研究的目的是确定在健康老年比格犬（n = 21）中，喂食富含抗氧化剂的食物以及环境/认知强化计划对所选炎症和免疫细胞离体检测的影响。使用2×2析因设计对四组犬进行测试。这项为期两年的纵向研究包括营养（对照食物或抗氧化剂强化食物）和行为（正常水平或认知强化）干预。行为强化包括增加运动量、环境强化和一系列学习任务。通过流式细胞术测量外周血中性粒细胞对调理素包被的乳胶珠的吞噬作用，发现同时接受膳食抗氧化剂和认知强化的犬的吞噬作用显著增加。与单独接受膳食抗氧化剂或认知强化的犬相比，用伴刀豆球蛋白A（Con A）同时刺激细胞并用地塞米松（Dex）抑制，导致同时接受膳食抗氧化剂和认知强化的犬的淋巴细胞增殖减少。在用Con A刺激淋巴细胞之前或之后，各组犬的CD4和CD8 T淋巴细胞亚群百分比之间没有显著差异。这些结果支持了我们的假设，即膳食抗氧化剂和行为强化均可增强宿主防御机制。
