Zouari Nacim, Bernadac Alain, Miled Nabil, Rebai Tarak, De Caro Alain, Rouis Souad, Carriere Frederic, Gargouri Youssef
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW route de Soukra, 3038 Sfax, Tunisia.
Biochim Biophys Acta. 2006 Sep;1760(9):1386-92. doi: 10.1016/j.bbagen.2006.02.009. Epub 2006 Mar 9.
The scorpion hepatopancreas consists of digestive diverticula and interstitial tissue. A digestive diverticulum is composed of two differentiated cell types: the secretory zymogene-like cells and the digestive cells which are the most abundant. The scorpion digestive lipase (SDL) has been previously purified from scorpion hepatopancreas, but its cellular localization has not yet been established. Polyclonal antibodies specific to SDL were prepared and used in immunofluorescence and immunogold techniques to determine the cellular location of SDL. Our results clearly established that SDL was detected intracellularly in specific vesicles tentatively named (SDL+) granules of the digestive cells. No immunolabelling was observed in secretory zymogene-like cells. This immunocytolocalization indicates that lipid digestion might occur in specific granules inside the digestive cells, as suggested by previous studies on the scorpion digestive process.
蝎子的肝胰腺由消化盲囊和间质组织组成。一个消化盲囊由两种分化的细胞类型组成：分泌型类酶原细胞和数量最多的消化细胞。蝎子消化脂肪酶（SDL）先前已从蝎子肝胰腺中纯化出来，但其细胞定位尚未确定。制备了针对SDL的多克隆抗体，并将其用于免疫荧光和免疫金技术，以确定SDL的细胞定位。我们的结果清楚地表明，在消化细胞中暂时命名为（SDL+）颗粒的特定囊泡内可在细胞内检测到SDL。在分泌型类酶原细胞中未观察到免疫标记。这种免疫细胞定位表明，正如先前关于蝎子消化过程的研究所暗示的那样，脂质消化可能发生在消化细胞内的特定颗粒中。
