Röll Sandra, Seul Judith, Paulsson Mats, Hartmann Ursula
Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany.
Matrix Biol. 2006 Aug;25(6):373-81. doi: 10.1016/j.matbio.2006.05.004. Epub 2006 May 23.
Testicans are proteoglycans belonging to the BM-40/SPARC/osteonectin family of extracellular calcium-binding proteins. Testican-1 is strongly expressed in the brain and has been reported to modulate neuronal attachment and matrix metalloproteinase activation. Characterization of the mouse testican-1 gene (Ticn1), consisting of 12 exons out of which exon 3 is alternatively spliced, allowed the construction of a gene targeting construct. Mice deficient in testican-1 showed no obvious morphological or behavioral abnormalities, were fertile, and had normal life spans. Despite the fact that neither of the testican-1 homologues expressed in the brain, testican-2, testican-3 and SC1/hevin, showed an increased expression in Ticn1 null mice, these results, together with those from other gene targetings, indicate extensive functional redundancy among brain proteoglycans.
睾丸蛋白聚糖是属于细胞外钙结合蛋白的BM-40/SPARC/骨连接蛋白家族的蛋白聚糖。睾丸蛋白聚糖-1在大脑中强烈表达，据报道它可调节神经元附着和基质金属蛋白酶激活。对由12个外显子组成的小鼠睾丸蛋白聚糖-1基因（Ticn1）进行表征，其中外显子3可选择性剪接，这使得构建基因靶向构建体成为可能。缺乏睾丸蛋白聚糖-1的小鼠没有明显的形态或行为异常，能生育，寿命正常。尽管在大脑中表达的睾丸蛋白聚糖-1的同源物睾丸蛋白聚糖-2、睾丸蛋白聚糖-3和SC1/纽蛋白均未显示在Ticn1基因敲除小鼠中有表达增加，但这些结果与其他基因靶向研究的结果一起表明，大脑蛋白聚糖之间存在广泛的功能冗余。
