Parrish Jodi R, Gulyas Keith D, Finley Russell L
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA.
Curr Opin Biotechnol. 2006 Aug;17(4):387-93. doi: 10.1016/j.copbio.2006.06.006. Epub 2006 Jun 27.
Interactome mapping, the systematic identification of protein interactions within an organism, promises to facilitate systems-level studies of biological processes. Using in vitro technologies that measure specific protein interactions, static maps are being generated that include many of the protein networks that occur in vivo. Most of the binary protein interaction data currently available was generated by large-scale yeast two-hybrid screens. Recent efforts to map interactions in model organisms and in humans illustrate the promise and some of the limitations of the two-hybrid approach. Although these maps are incomplete and include false positives, they are proving useful as a framework around which to elaborate and model the in vivo interactome.
相互作用组图谱绘制，即对生物体中蛋白质相互作用进行系统鉴定，有望推动对生物过程的系统层面研究。利用测量特定蛋白质相互作用的体外技术，正在生成包含许多体内存在的蛋白质网络的静态图谱。目前可用的大多数二元蛋白质相互作用数据是通过大规模酵母双杂交筛选产生的。最近在模式生物和人类中绘制相互作用图谱的努力说明了双杂交方法的前景和一些局限性。尽管这些图谱并不完整且包含假阳性，但它们正被证明是一个有用的框架，围绕该框架可以阐述和模拟体内相互作用组。
