颈部淋巴结制备：一种通过活体显微镜研究淋巴细胞归巢的新方法。
The cervical lymph node preparation: a novel approach to study lymphocyte homing by intravital microscopy.
作者信息
Schramm R, Schäfers H-J, Harder Y, Schmits R, Thorlacius H, Menger M D
机构信息
Institute for Clinical & Experimental Surgery, Department of Thoracic and Cardiovascular Surgery, University Hospitals of Saarland, Kirrberger Strasse, 66421 Homburg/Saar, Germany.
出版信息
Inflamm Res. 2006 Apr;55(4):160-7. doi: 10.1007/s00011-006-0066-0.
OBJECTIVE
Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo.
METHODS AND RESULTS
Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92% and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%. In addition to the microcirculation studies, the cervical lymph node preparation allowed for visualization of afferent lymphatic transport, which is mainly derived from the oral mucosa.
CONCLUSION
This study reports a novel technical tool for the detailed in vivo analysis of adaptive immune responses.
目的
淋巴细胞再循环是适应性免疫系统的一个组成部分。血源性淋巴细胞迁移至二级淋巴器官，穿过位点特异性高内皮微静脉（HEV）的血管壁。我们制备了小鼠颈部淋巴结制剂，以研究体内淋巴细胞归巢。
方法与结果
我们的新方法通过活体荧光显微镜对血流动力学和淋巴细胞与HEV内皮的相互作用进行了详细分析。我们证实了L-选择素和淋巴细胞功能相关抗原-1（LFA-1）在淋巴细胞归巢中的关键作用。L-选择素功能的阻断使淋巴细胞滚动和牢固黏附分别受到92%和66%的抑制。在LFA-1缺陷小鼠中，淋巴细胞牢固黏附减少了70%。除了微循环研究外，颈部淋巴结制剂还能观察到主要源自口腔黏膜的传入淋巴运输。
结论
本研究报告了一种用于详细体内分析适应性免疫反应的新型技术工具。
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