Wu Suh-Chin, Yu Jung-Chuan, Hsu Shan-Hui, Chen David Chanhen
Institute of Biotechnology, Department of Life Science, National Tsing-Hua University, Hsinchu, Taiwan.
J Biomed Mater Res A. 2006 Dec 1;79(3):557-65. doi: 10.1002/jbm.a.30845.
Through the recombinant DNA technology, it is possible to create artificial extracellular matrix (aECM) proteins with domains chosen to modulate cellular behaviors. In this study, we constructed three aECM proteins containing both heparin-binding and RGD-containing domains (387RGDS, Tri-FN10, and TNC-FN3) produced in Escherichia coli. Promotion of MG-63 cell attachment and growth in two-dimensional (2D) cultures (tissue culture plate, polycaprolactone membrane) and 3D cultures (Cytodex 1 and Plastic Plus microcarriers) were demonstrated on these three aECM protein-coated surfaces. These three aECM proteins improved MG-63 cell attachment and growth in the order TNC-FN3 > Tri-FN10 > 387RGDS in both 2D and 3D cultures. This study is the first report of the construction of aECM proteins that contain both heparin-binding and RGD-containing domains used in osteoblast tissue engineering applications.
通过重组DNA技术，可以创建具有选定结构域以调节细胞行为的人工细胞外基质（aECM）蛋白。在本研究中，我们构建了三种在大肠杆菌中产生的含有肝素结合结构域和含RGD结构域的aECM蛋白（387RGDS、Tri-FN10和TNC-FN3）。在这三种aECM蛋白包被的表面上，证实了其在二维（2D）培养（组织培养板、聚己内酯膜）和三维（3D）培养（Cytodex 1和Plastic Plus微载体）中促进MG-63细胞附着和生长的作用。在二维和三维培养中，这三种aECM蛋白促进MG-63细胞附着和生长的能力顺序为TNC-FN3 > Tri-FN10 > 387RGDS。本研究首次报道了构建用于成骨细胞组织工程应用的同时含有肝素结合结构域和含RGD结构域的aECM蛋白。
