Kobayashi Shiro, Ohmae Masashi, Ochiai Hirofumi, Fujikawa Shun-Ichi
Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Chemistry. 2006 Aug 7;12(23):5962-71. doi: 10.1002/chem.200600191.
Hyaluronidase (HAase) catalyzes multiple enzymatic polymerizations with controlling regio- and stereoselectivity perfectly. This behavior, that is, the single enzyme being effective for multireactions and retaining the enzyme catalytic specificity, is not usual, and hence, HAase is a supercatalyst. Various sugar oxazoline monomers prepared based on the concept "transition-state analogue substrate" were successfully polymerized and copolymerized with HAase catalysis, yielding natural and unnatural glycosaminoglycans.
透明质酸酶（HAase）能完美地催化多种具有区域和立体选择性控制的酶促聚合反应。这种行为，即单一酶对多种反应有效并保留酶催化特异性，并不常见，因此，HAase是一种超级催化剂。基于“过渡态类似物底物”概念制备的各种糖恶唑啉单体在HAase催化下成功实现了聚合和共聚，生成了天然和非天然的糖胺聚糖。
